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基于Rnai的基因的功能分析,这些基因与蝶甲虫的皮肤色素有关

Lan-Lan Liao¹, Wen-Ze Li¹, Lin Jin¹

¹Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests/State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.

Journal of insect physiology

|August 22, 2024

概括

布尔西康信号调节蝶甲虫Henosepilachna vigintioctomaculata的皮肤质颜色,通过影响黑色素和硬质素的比率. 这一途径在发育过程中对身体色彩至关重要.

科学领域:

昆虫学昆虫学是一门学科.
发展生物学发展生物学
分子信号传输的方法

背景情况:

布尔西康信号传递,涉及布尔西康异构体/同构体和LGR2受体,控制生理过程,如节肢动物的皮肤色素化.
28个斑点较大的土豆,Henosepilachna vigintioctomaculata,为研究昆虫色素机制提供了一个模型.

研究的目的:

为了调查bursicon信号是否调解了Henosepilachna vigintioctomaculata的身体颜色.
为了阐明bursicon信号调节这种母甲虫颜色的机制.

主要方法:

在不同生命阶段对Hvburs,Hvpburs和Hvrk的基因表达分析.
通过RNA干扰 (RNAi) 在幼虫前的Hvburs,Hvpburs或Hvrk被击落.
对表型缺陷的分析,特别是胸部色素的分析.
测量mRNA水平的硬质素生物合成基因 (Hvadc,Hvebony) 的量化.
通过减少RNAi甲虫中的Hvadc或Hvebony进行救援实验.

主要成果:

在幼虫,和成年人中观察到Hvburs,Hvpburs和Hvrk的高表达,特别是在头部和腹部神经中.
敲除Hvburs,Hvpburs或Hvrk导致成年甲甲虫的胸部色素受损.
RNAi导致Hvadc和Hvebony的mRNA水平增加,这些基因是结核素合成中的关键基因.
耗尽Hvadc或Hvebony可以挽救Bursicon耗尽的甲虫的色素缺陷.

结论:

布尔西康的异构体介导信号调节了Henosepilachna vigintioctomaculata中的皮质颜色.
这种信号通路可能会调节黑色素和硬质素之间的平衡,以控制胸骨颜色.

关键词:

布尔西康的信号传递.黑色素是黑色素的一种.颜色化颜色化是一种颜色.斯克莱罗丁是一种硬质素.骨是什么意思骨是什么意思

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