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[高产糖酸菌株的选和发酵]

Qingqing Bao^1,2, Guang Yang^1,2, Feifei Chen^1,2

¹Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China.

Sheng wu gong cheng xue bao = Chinese journal of biotechnology

|August 22, 2024

概括

研究人员开发了一种基因稳定的Rhodotorula菌株,用于使用乙烯糖醇和葡萄糖高效生产甘油酸. 这种方法克服了当前微生物生产的局限性,为具有成本效益的工业应用铺平了道路.

科学领域:

生物技术是生物技术.
工业微生物学工业微生物学
代谢工程是代谢工程.

背景情况:

微生物生产甘醇酸面临的挑战包括低产量,遗传不稳定性和高成本.
目前的全细胞催化方法通常需要昂贵的碳来源,如比醇,阻碍了工业的可扩展性.

研究的目的:

开发一种可行工业化方法来生产甘氨酸.
选和设计一种强大的微生物菌株,能够利用廉价的碳来源进行甘醇酸生物合成.

主要方法:

使用乙烯糖醇作为基质通过全细胞催化产生甘油酸的菌株的选.
紫外线突变和高通量查以获得增强的突变菌株 (RMGly-20).
优化发酵条件,包括在5L发酵器中使用葡萄糖作为碳源的料批培养.

主要成果:

一种新型的生产甘油酸的Rhodotorula sp. 已经确定了菌株.
经过优化后,工程菌株RMGly-20的甘油酸产量 (17.8 g/L) 增加了10.1倍.
使用葡萄糖的料批发发酵产生了相当大的61.1 g/L的甘油酸.

结论:

成功培育出一种基因稳定的Rhodotorula菌株,以节省成本,生产高效的糖果酸.
开发的菌株和工艺为工业规模的葡萄糖酸生物合成提供了一个有希望的替代方案.
这项研究提供了一个新的微生物平台,以推进甘醇酸的工业生产.

关键词:

罗多托鲁拉 (Rhodotorula sp.) 是一种的植物.紫外线变异的发生乙烯基甘醇是一种乙烯基甘醇.葡萄糖酸是什么葡萄糖酸是什么高吞吐量选的高吞吐量选

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