中性粒细胞中的Piezo1表达调节了剪切诱导的NETosis
Sara Baratchi1,2,3, Habiba Danish4, Chanly Chheang5
1Baker Heart and Diabetes Institute, Melbourne, VIC, 3004, Australia. sara.baratchi@baker.edu.au.
Nature communications
|August 22, 2024
概括
剪切应激激活中性粒细胞中的Piezo1通道,触发细胞内的增加和细胞骨的变化,导致中性粒细胞外陷形成 (NETosis),导致炎症和血栓形成.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 中性细胞外细胞陷形成 (NETosis) 导致无菌炎症和血栓形成.
- 中性粒细胞在循环中经历血动力学力量,如切割应力,影响它们的功能.
- 在中性粒细胞中机械转导的机制仍然不完全理解.
研究的目的:
- 研究剪切应力在中性粒细胞外细胞陷形成 (NETosis) 中的作用.
- 阐明中性粒细胞中剪切应力诱导的NETosis背后的分子机制.
主要方法:
- 将中性粒细胞暴露在不同程度的剪切应力下.
- 测量细胞内水平.
- 评估Piezo1通道和calpain活动.
- 对细胞骨重塑和NETosis诱导的分析.
主要成果:
- 切割应力以水平依赖的方式诱导NETosis.
- 剪切应激增强中性粒细胞对NETosis诱导剂的敏感性.
- 剪切应力通过机械敏感离子通道Piezo1.1增加细胞内.
- 皮埃佐1激活调解了calpain活动和细胞骨重塑,导致NETosis.
结论:
- 剪切应激是中性粒细胞内 NETosis 的直接诱导因素.
- Piezo1-calpain-cytoskeleton轴是剪切应力介导NETosis的一个关键途径.
- 这条通路将机械力量与由中性粒细胞介导的炎症和前血栓反应联系起来.
更多相关视频
11:03High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro
Published on: June 18, 2016
13.1K
09:20The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
Published on: October 31, 2016
8.1K
相关概念视频
Mechanism of Filopodia Formation
2.3K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.3K
Cell Polarization by Rho Proteins
2.7K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
2.7K
Cell Motility through Blebbing
1.9K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
1.9K
Mechanism of Lamellipodia Formation
2.5K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.5K
Cell-matrix's Response to Mechanical Forces
2.6K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...
Anchoring junctions mechanically attach a cell to the...
2.6K
