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Updated: Jun 15, 2025

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
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Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking

Published on: December 17, 2013

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PglX甲基转移酶的结构和合理工程以及BREX菌体防御的特异性因子

Sam C Went1, David M Picton1, Richard D Morgan2

  • 1Department of Biosciences, Durham University, South Road, Durham, UK.

Nature communications
|August 22, 2024
PubMed
概括

细菌使用菌体排除 (BREX) 系统来防御病毒. 这项研究揭示了BREX蛋白PglX如何识别特定的DNA序列,从而实现更广泛的菌体防御和宿主甲基化能力.

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Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

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An Assay for Quantifying Protein-RNA Binding in Bacteria07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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