相关实验视频

Updated: Jun 15, 2025

Author Spotlight: Exploring Photodynamic Therapy with Curcumin in a Murine Model for Oral Candidiasis

Author Spotlight: Exploring Photodynamic Therapy with Curcumin in a Murine Model for Oral Candidiasis

Published on: October 27, 2023

含有克洛特里马的微生物杀伤性聚合物纳米颗粒用于治疗vulvovaginal候群病

María Del Rocío Lara-Sánchez¹, Adriana Ganem-Rondero², María Guadalupe Nava-Arzaluz²

¹Laboratorio de Sistemas Farmacéuticos de Liberación Modificada (L-13, UIM), Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, 54714, Estado de México, Mexico.

AAPS PharmSciTech

|August 22, 2024

概括

新的纤维素甲 (CAP) 和克洛特里马 (CLZ) 纳米颗粒有效地抑制了Candida albicans的生长,为vulvovaginal candidiasis (VVC) 提供了有前途的治疗方法. 这些纳米粒子提供持续的药物释放,并显示出杀菌效应.

科学领域:

制药科学制药科学
生物材料科学生物材料科学
菌类学菌类学是指菌类学.

背景情况:

Vulvovaginal candidiasis (VVC) 损害了宫性阴道免疫力,持续发病超过30年,需要新的治疗策略.
由于疾病发病率不变,目前对VVC的治疗需要改进.
开发先进的药物输送系统对于有效的VVC管理至关重要.

研究的目的:

开发和描述用于VVC处理的纤维素甲 (CAP) 和克洛特里马 (CLZ) 纳米颗粒.
评估开发的纳米颗粒的体外释放动力学,粘膜粘合和抗真菌功效.
在加速条件下评估CAP-CLZ纳米粒子的物理稳定性.

主要方法:

使用乳化-扩散方法制备纳米颗粒,并通过动态光散射,原子力显微镜和差分扫描热量计来表征.
使用透析袋技术研究了体外药物释放,并通过粘素颗粒方法评估了粘粘度.
通过盘子计数确定对Candida albicans的抗真菌活性,并在水性介质和模拟阴道液 (SVF) 中测试物理稳定性.

主要成果:

CAP-CLZ纳米颗粒的平均直径为273.4nm,PDI为0.284,表面光滑,形状球形.
在体外释放研究表明,从CAP矩阵中持续释放CLZ,符合韦布尔模型.
与自由的CLZ相比,CAP-CLZ纳米颗粒显示出对C. albicans生长的增强抑制,CAP-CLZ纳米颗粒显示出一种杀菌效应.

结论:

成功开发了一种结合CLZ和CAP的新型纳米颗粒系统,提供持续的抗真菌药物递送.
开发的纳米颗粒对Candida albicans表现出显著的抗真菌活性,可能改善VVC治疗结果.
进一步调查这些纳米颗粒的粘液透能力是有必要的,尽管观察到的粘液粘性差.

关键词:

在Candida albicans中使用.纤维素乙烯酸甲酸是什么纤维素乙烯酸克洛特里马 (Clotrimazole) 是一种药物.聚合物纳米粒子聚合物纳米粒子 Vulvovaginal 候选症的发生

更多相关视频

Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis

Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis

Published on: December 8, 2011

A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms

A Soluble Tetrazolium-Based Reduction Assay to Evaluate the Effect of Antibodies on Candida tropicalis Biofilms

Published on: September 16, 2022

相关概念视频

Surface Membrane Barriers

Surface Membrane Barriers

The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...

Hand hygiene

Hand hygiene

Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
Hand washing...