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Updated: May 10, 2026

The MultiBac Protein Complex Production Platform at the EMBL
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多重表达卡塞特组装:一种灵活多功能的方法,用于在传统载体中构建复杂的遗传电路

Xun Jiang1, Zhuoxiang Zhang1, Xiuming Wu1

  • 1Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, China.

Plant biotechnology journal
|August 23, 2024
PubMed
概括

多重表达卡塞特组装 (MECA) 简化了为合成生物学和作物改进创建复杂的遗传电路. 这种高效的方法使得协调基因表达成为可能,加速了先进作物的发展.

关键词:
黄金大门大会的会议会议.代谢工程是代谢工程.多重表达卡塞特多重表达卡塞特.合成生物学 合成生物学转基因植物转基因植物

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

Overview
Design Example: Capacitance Multiplier Circuit01:20

Design Example: Capacitance Multiplier Circuit

In integrated circuit technology, a capacitance multiplier is often utilized to produce a larger capacitance value when a small physical capacitance falls short. This is achieved by a circuit that multiplies capacitance values by a factor of up to 1000, such that a 10-pF capacitor can replicate the performance of a 100-nF capacitor.
The circuit illustrated in Figure 1 below incorporates two op-amps, with the first operating as a voltage follower and the second acting as an inverting amplifier.