蛋白质组学分析揭示了 oogenesis 期间的脂质代谢异常在无法解释的重复性妊娠损失中
Kun Liu1,2, Xiaojuan Xu1, Liang Sun1
1Reproductive Medicine Center, The First Hospital of Lanzhou University, Lanzhou, Gansu, China.
Frontiers in immunology
|August 23, 2024
概括
无法解释的重复妊娠损失 (URPL) 与源自 oogenesis 的免疫和凝血通路中断有关. 毛囊液和血液中的下方阿波利波蛋白B (APOB) 表明URPL中脂蛋白失调的作用.
科学领域:
- 生殖医学 生殖医学
- 蛋白质组学是指蛋白质组学.
- 免疫学 免疫学 免疫学
背景情况:
- 无法解释的重复妊娠损失 (URPL) 在生殖医学中构成了重大临床挑战.
- 诊断往往是排斥性的,尽管进行了彻底的评估,但一些患者仍然存在流产的高风险.
- 研究的URPL患者中没有内分泌异常或其他可验证的原因.
研究的目的:
- 与对照组相比,确定URPL患者的毛囊液 (FF) 中的差异表达蛋白 (DEPs).
- 调查与URPL相关的生物途径和蛋白质-蛋白质相互作用 (PPI).
- 探索URPL.的潜在生物标志物和治疗点.
主要方法:
- 使用从URPL患者和对照对FF的直接数据独立获取 (dDIA) 的定量蛋白质组学.
- 生物信息学分析使用g:profiler,String和ToppGene进行丰富分析.
- 使用Cytoscape构建蛋白质与蛋白质相互作用 (PPI) 网络,并通过ELISA进行验证.
主要成果:
- 基因实体学和KEGG分析揭示了DEP参与补充和凝血级联.
- 脂蛋白 (APOs) 成为PPI网络中的关键蛋白质.
- 艾利萨检测证实,URPL患者的FF和外周血液中阿波利波蛋白B (APOB) 的表达显著降低.
结论:
- URPL的特点是免疫和凝血网络失调,从 oogenesis 阶段就存在不平衡.
- 异常的脂蛋白调节,以低APOB表示,是导致URPL的潜在关键因素.
- 这些发现凸显了早期干预的必要性,并为URPL提出了新的治疗目标.
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