神经内分泌细胞在鼻子粘膜中的罕见存在和功能
Tine Wils1, Wout Backaert1,2, Inge Jacobs1,3
1KU Leuven Department of Microbiology, Immunology and Transplantation, Allergy and Clinical Immunology Research Group, KU Leuven, Leuven, Belgium.
Frontiers in immunology
|August 23, 2024
概括
神经内分泌细胞 (NEC) 在鼻子粘膜中没有显著存在,并且与鼻子过敏反应 (NHR) 或上呼吸道疾病的严重程度无关. 这质疑它们在鼻腔病理学中的作用.
科学领域:
- 免疫学 免疫学 免疫学
- 耳鼻喉科 耳鼻喉科 耳鼻喉科
- 细胞生物学 细胞生物学
背景情况:
- 神经性炎症与上呼吸道疾病有关,鼻腔过敏反应 (NHR) 是一个关键症状.
- 神经内分泌细胞 (NEC) 与支气管和肠道的高反应性有关,但它们在鼻子粘膜中的存在和作用尚不清楚.
研究的目的:
- 调查NEC在健康个体和患有过敏性鼻炎和长期鼻炎与鼻的患者鼻上皮质中的存在和潜在作用.
- 为了确定NEC是否与鼻腔过敏反应 (NHR) 或疾病严重程度相关.
主要方法:
- 在人类鼻腔组织和细胞培养中使用RT-qPCR,西部斑,免疫组织化学,RNA范围,流细胞计和单细胞RNA测序来分析NEC标记物 (CHGA,ASCL1,CGRP).
- 通过使用视觉模拟尺度得分来评估NHR通过冷干空气挑和疾病严重程度.
主要成果:
- 在鼻组织和细胞培养中发现NEC标记物的有限基因和蛋白质表达 (CHGA,ASCL1),与NHR或疾病严重程度无关.
- 在鼻子活检中,CHGA是无法检测的,而不是在骨样本中.
- 单细胞RNA测序没有在上呼吸道组织中确定一个独特的NEC集群.
结论:
- 在鼻腔粘膜中存在NEC的证据有限.
- NEC似乎没有显著地参与NHR或上呼吸道疾病的病理生理学.
- 在上呼吸道病理中NEC的相关性是可疑的.
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