在牛产后子宫内膜炎上表达牛粒细胞 - 巨细胞殖民地刺激因子的工程Lactobacillus johnsonii的保护作用
Jing Guo1,2,3, Xu Cao1,2,3, Zhiqiang Li1,2,3
1Key Lab of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, China.
Frontiers in veterinary science
|August 23, 2024
概括
经过工程设计的表达GM-CSF的Lactobacillus johnsonii通过降低炎症性细胞因子,减少了牛的产后子宫炎症. 这种新型益生菌为牛子宫内膜炎提供了有前途的治疗方法,并改善了生殖健康.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
背景情况:
- 产后子宫内膜炎是一种常见的牛生殖问题,导致不孕.
- 乳酸菌株可以减少子宫炎症,但有局限性.
研究的目的:
- 为了设计一种表达牛肉花细胞巨细胞殖民地刺激因子 (GM-CSF) 的再组合Lactobacillus johnsonii菌株.
- 评估这种工程菌株在减轻牛的产后子宫炎症方面的疗效.
主要方法:
- 再组合的L. johnsonii表达GM-CSF给孕小鼠.
- 产后子宫内膜炎是通过大肠杆菌感染引起的.
- 测量了炎症标志物 (IL-6,IL-1β,TNF-α),MPO活性和NO度. 评估了子宫组织学.
主要成果:
- 重组的GM-CSF产生的L. johnsonii显著降低了子宫炎症标志物.
- 组织学分析显示,子宫形态改善,损伤减少.
- 该菌株通过降低炎症性细胞因子,对临床牛子宫内膜炎产生有益影响.
结论:
- 再组合的L. johnsonii表达GM-CSF提供了对牛的产后子宫内膜炎的保护作用.
- 这种工程化益生菌菌株为牛生殖障碍提供了一种新的治疗策略.
- 这些发现表明,有可能提高牛的生殖效率.
相关概念视频
01:22Probiotics
296
Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...
296
01:25Clinical Significance of Antibiotic Resistance
90
Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
90