系统工程是为了提高酵母酵母中的β-米尔生产
Yujie Shu1,2, Tianyu Dong1,2, Xiao Zhou1,2
1School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Journal of agricultural and food chemistry
|August 23, 2024
概括
在酵母中,微生物对β-米尔的产生显著增强. 包括酶工程和前体供应在内的策略将产量提高到创纪录的142.64毫克/升,为绿色制造铺平了道路.
科学领域:
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
- 生物技术是生物技术.
背景情况:
- 贝塔-米尔是一种有价值的单烯,用于香水,农业和食品工业.
- 微生物生产提供了一个可持续的替代方案,以传统的方法用于β-米尔合成.
- 主要挑战包括低β-米尔合成酶活性和有限的前体可用性.
研究的目的:
- 开发一个高效的微生物生产平台,使用Saccharomyces cerevisiae来生产β-myrcene.
- 通过综合代谢工程策略,克服β-米尔生物合成的瓶.
- 为了实现工业应用的酵母中高度β-米尔的生产.
主要方法:
- 综合源查,亚细胞定位,酶融合和前体增强策略.
- 优化beta-myrcene合成酶 (MS) 活动和前体供应途径.
- 用碳限制和添加抗氧化剂 (谷氨和BHT) 的料批发发酵.
主要成果:
- 实现了218倍的β-米尔标位增加,在摇瓶中达到63.59 mg/L.
- 补充抗氧化剂 (GSH和BHT) 增加了标位至66.82 mg/L.
- 在5.0L料批发发酵中达到142.64 mg/L的最高标位.
结论:
- 成功建立了一个强大的酵母平台,用于生产高位β-米尔.
- 证明了综合策略在克服生物合成局限性的有效性.
- 为微生物合成其他有价值的单烯提供了宝贵的参考.
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