相关实验视频
Updated: Jun 15, 2025

14:08
Study of Protein-protein Interactions in Autophagy Research
Published on: September 9, 2017
9.1K
肌管素2与SEC23A相互作用,并在Arabidopsis中负面调节ER退出部位的自
Xinjing Li1, Jing Zheng2, Jing Su2
1State Key Laboratory of Microbial Metabolism & Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, P. R. China.
Autophagy
|August 23, 2024
概括
阿拉比多普西斯中的Myotubularin 2 (MTM2) 作为ER退出站点的酸酶,调节自和COPII囊泡贩运. 这一发现揭示了对自细胞形成和应激反应至关重要的酸周转的平衡.
科学领域:
- 细胞生物学 细胞生物学
- 分子植物科学 分子植物科学
- 自学研究 自学研究
背景情况:
- 脂 3-酸盐 (PtdIns3P) 在内分泌网膜 (ER) 的产生对自细胞形成至关重要.
- 在ER退出部位 (ERES) 中PtdIns3P酸酶在调节自的作用仍然未被探索.
研究的目的:
- 确定和描述在ERES中运行的PtdIns3P酸酶.
- 为了研究Myotubularin 2 (MTM2) 在Arabidopsis的自和ER相关过程中的作用.
主要方法:
- 在MTM2的体外结合试验和体内定位研究.
- 分析MTM2突变体和过度表达系中的自流量,蛋白质积累和应激耐受性.
- 研究MTM2与自相关蛋白 (ATG) 和COPII组件的相互作用 (SEC16,SEC23A).
主要成果:
- 阿拉比多普西斯MTM2被确定为ERES局部化的PtdIns3P酸酶,可以负面调节自.
- MTM2过度表达抑制了自流和蛋白质分泌,而mtm2突变体表现出增强的自和饥饿耐受性.
- 具体而言,MTM2在ERES中发挥作用,与COPII蛋白相互作用并影响盐应激反应.
结论:
- 在ERES中,MTM2在平衡PtdIns3P水平方面发挥着关键作用,从而控制自细胞形成.
- 在自调节和COPII囊泡贩运之间建立了一个新的联系.
- 在饥饿和盐压力期间,MTM2有助于细胞适应.
相关概念视频
Tail-anchoring of Proteins in the ER Membrane
3.1K
Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...
3.1K
Post-translational Translocation of Proteins to the RER
5.6K
A sizable fraction of proteins destined for ER are first synthesized in the cell cytosol and then transported across the ER membrane–a process called post-translational translocation. Similar to cotranslationally translocated proteins, these proteins also use the Sec translocon complex to enter the ER lumen.
Targeting proteins to the ER
Hsp40 and Hsp70 chaperone molecules bind the translated proteins in the cytosol to prevent their folding. The chaperone binding helps to keep the signal...
Targeting proteins to the ER
Hsp40 and Hsp70 chaperone molecules bind the translated proteins in the cytosol to prevent their folding. The chaperone binding helps to keep the signal...
5.6K
Export of Misfolded Proteins out of the ER
3.5K
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
3.5K
Microtubule Associated Motor Proteins
7.7K
Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular...
7.7K
Cotranslational Protein Translocation
7.3K
Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
7.3K
ER Retrieval Pathway
3.8K
In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
3.8K

