在它在合成中的作用之前,RNA氨基化具有潜在的作用
Aleksandar Radakovic1,2, Anna Lewicka3, Marco Todisco1
1HHMI, Department of Chemistry, The University of Chicago, Chicago, IL 60637.
概括
原始RNA可以非酶性氨基酸和结合,形成氨基酸桥接RNA结构. 这一发现表明,在编码蛋白质合成进化之前,早期RNA功能存在一个途径.
科学领域:
- 生命起源研究研究生命的起源.
- RNA生物化学与RNA生物化学
- 分子进化是分子进化的过程.
背景情况:
- 编码蛋白质合成的进化需要先前存在的氨基酸转移RNA (tRNA).
- 氨基基酸RNAs的原始功能仍然是一个开放的问题.
研究的目的:
- 调查氨基酸RNA是否可能在蛋白质合成之前发挥作用.
- 探索氨基酸纳入RNA的非酶途径.
主要方法:
- 深度测序以确定经过高效的甘氨酸氨基化和结合的RNA序列.
- 确定糖氨酸桥接RNA针头的晶体结构.
- 展示了T-循环辅助组装的仿真氨基酸-RNA合成酶 ribozymes.
主要成果:
- 特定的RNA序列可以非酶性氨基化和结合,以形成氨基酸桥接的干环RNA.
- 一个用甘氨酸桥接的RNA发针表现出类似于现代tRNAs的T-循环结构.
- 通过T-循环辅助的氨基酸桥接,可以促进非模板式的嵌合式 ribozymes 的组装.
结论:
- 氨基酸桥接RNA结构代表了氨基酸RNA的可信的原始功能.
- 这一过程为共价氨基酸纳入RNA提供了一条简单的途径,增强了 ribozyme 的功能.
- 这些发现为RNA世界中的编码蛋白质合成提供了潜在的进化步骤.
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