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相关概念视频

Epigenetic Regulation01:46

Epigenetic Regulation

31.0K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
31.0K
Histone Modification02:32

Histone Modification

13.2K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
13.2K
Heterochromatin02:38

Heterochromatin

11.8K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
11.8K
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

4.3K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.3K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

6.2K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
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Master Transcription Regulators02:23

Master Transcription Regulators

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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相关实验视频

Updated: Jun 15, 2025

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
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Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

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CDCA7是一种进化保存的半甲基化DNA传感器,存在于真核生物中.

Isabel E Wassing1, Atsuya Nishiyama2, Reia Shikimachi3

  • 1Laboratory of Chromosome and Cell Biology, The Rockefeller University, New York, NY 10065, USA.

Science advances
|August 23, 2024
PubMed
概括

CDCA7-HELLS复合体独特地感知半甲基化DNA,这对于维持DNA甲基化模式至关重要. 这一发现解释了它在预防DNA低甲基化和相关遗传疾病方面的作用.

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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
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Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina
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Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina

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相关实验视频

Last Updated: Jun 15, 2025

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
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Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

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Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina
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科学领域:

  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个

背景情况:

  • 在HELLS和CDCA7中发生的突变会导致DNA低甲基化综合征.
  • CDCA7-HELLS维持DNA甲基化的确切机制尚不清楚.
  • 这种复合体是唯一已知的核体重塑剂,其缺乏会破坏DNA甲基化维护.

研究的目的:

  • 阐明CDCA7-HELLS复合体维持DNA甲基化的机制.
  • 为了确定 CDCA7 参与 DNA 甲基化维护的独特功能域.
  • 了解CDCA7-HELLS如何与DNA甲基化通路中的其他关键蛋白相互作用.

主要方法:

  • 蛋白质域的识别和表征.
  • 低温电子显微镜用于对CDCA7-核体复合体进行结构分析.
  • 生物化学试验用于研究蛋白质-DNA相互作用和染色质修饰.

主要成果:

  • 在CDCA7 (HMZF) 中确定了一个独特的指域,该域感知半甲基化CpG位点.
  • 结构分析显示,HMZF能够在核子体内识别半甲基化DNA,而UHRF1无法做到这一点.
  • 证明CDCA7对半甲基化染色质招募HELLS,促进UHRF1-介导的H3无处不在和DNA甲基化.

结论:

  • CDCA7-HELLS复合体作为DNA甲基化维护的关键调节者.
  • CDCA7的HMZF域可识别不可访问的半甲基化CpG位点,确保表观遗传稳定性.
  • 这种机制对于预防DNA低甲基化和相关遗传疾病至关重要.