完全集成的识别和丰富电子喷雾电离源用于高灵敏度质谱测量生物胺的确定
Weilai Wang1,2,3, Tong Liu1,2, Xiujuan Wang1,2
1Institute of Food Safety, Chinese Academy of Inspection & Quarantine, Beijing 100176, China.
Analytical chemistry
|August 23, 2024
概括
研究人员开发了一种使用分子印记纳米纤维的新型电子喷雾电离源,用于在复杂样品中快速检测有害生物胺 (希斯胺,胺,胺).
科学领域:
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
背景情况:
- 使用环境质谱 (AMS) 在复杂样品中快速检测有害物质的痕迹,在实现高特异性方面面临挑战.
- 开发特定的识别电喷射电离 (ESI) 源对于有效的AMS分析至关重要.
研究的目的:
- 构建一个分子印记纳米纤维电膜涂层钢基板 (MINMCS) 作为AMS的高度特定的识别和丰富ESI源模块.
- 评估MINMCS在生物胺 (BA) 快速检测方面的性能.
主要方法:
- 使用电策略制造MINMCS,采用分子印记纳米纤维膜进行提取和丰富.
- 使用MINMCS作为ESI源用于AMS检测组胺素 (HIS),硫胺素 (TYR) 和胺素 (TRY).
- 通过简化样品预处理来评估负载能力,灵敏度和回收率.
主要成果:
- 对于目标生物胺,MINMCS显示出足够的载荷能力.
- 开发的电离源的灵敏度与HPLC-MS/MS相提并论.
- 整个分析过程在1分钟内完成,可接受的恢复率 (83.21-101.80%).
结论:
- 一个新的综合识别和丰富ESI源 (MINMCS) 已成功开发用于检测有害物质,如生物胺.
- 这项技术显示出重要的商业潜力,用于快速检测食源性有害化合物.
- MINMCS为复杂样品的敏感和快速AMS分析提供了一个有希望的解决方案.
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