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Updated: Jun 15, 2025

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多光子光显微镜用于体内成像

Chris Xu1, Maiken Nedergaard2, Deborah J Fowell3

  • 1School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14850, USA.

Cell
|August 23, 2024
PubMed
概括

多光子光显微镜 (MPFM) 通过克服光散射,使高分辨率的深层组织成像成为可能. 这种多功能技术对于研究神经科学,癌症生物学和免疫学中的生物过程至关重要.

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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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