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由于神秘的Aspergillus物种引起的侵入性阿斯伯吉洛症:来自印度单一中心的前性研究

R Sruti Janani¹, Immaculata Xess¹, Bimal Kumar Das¹

¹All India Institute of Medical Sciences, New Delhi, India.

Indian journal of medical microbiology

|August 23, 2024

概括

隐秘的阿斯伯吉卢斯物种,在视觉上是相同的,但在分子上是不同的,这带来了诊断挑战. 这项研究使用分子技术在临床样本中确定了两个神秘物种Aspergillus stellatus和Aspergillus tubingensis.

科学领域:

医学真菌学医学真菌学
分子诊断学分子诊断
抗微生物耐药性抗微生物耐药性

背景情况:

由于不同的抗真菌易感性,视觉上相同的神秘阿斯伯吉洛斯物种需要分子识别.
准确的鉴定对于适当的患者治疗和管理至关重要.
隐秘的物种往往表现出较高的抗真菌药物的最小抑制度.

研究的目的:

从临床样本中识别和描述神秘的Aspergillus物种.
突出分子方法在区分神秘的阿斯伯吉卢斯物种中的重要性.
为了有助于更好地了解与阿斯伯吉勒斯菌相关的感染.

主要方法:

从临床样本中分离的阿斯伯吉路斯菌的分析,使用表型识别和矩阵辅助激光溶解电离 - 飞行时间 (MALDI-TOF).
通过聚合酶链反应 (PCR) 和内部转录间隔器 (ITS) /β-tubulin基因测序来对神秘物种进行分子鉴定.
根据CLSI指南,使用微稀释进行的抗真菌敏感性测试.

主要成果:

形态鉴定揭示了常见的阿斯伯吉洛斯物种,但MALDI-TOF错误识别了一些孤立物种.
分子分析,特别是β-蛋白测序,确定了两种神秘物种:Aspergillus stellatus和Aspergillus tubingensis (2.08%的分离物).
这凸显了某些Aspergillus物种的表型和MALDI-TOF识别的局限性.

结论:

像β-蛋白测序这样的分子技术对于准确识别神秘的阿斯伯吉卢斯物种至关重要.
鉴定Aspergillus stellatus和Aspergillus tubingensis强调了临床微生物学中分子诊断的需要.
区分神秘物种对于指导抗真菌疗法和改善患者治疗结果至关重要.

关键词:

阿斯珀吉勒斯神秘的物种亚斯伯吉勒斯 (Aspergillus stellatus) 是一种的植物.亚斯伯菌管道感应感应 ITS和β-tubulin中的一个.马尔迪 - 托夫

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