自粘性聚氨酸/酸混合水凝,具有对生物膜的协同抗菌活性
Deepa Garg1, Deepak Kumar1, Sakshi Paliwal2
1Materials Science & Sensor Application Division, CSIR-Central Scientific Instruments Organisation (CSIR-CSIO), Chandigarh 160030, India; Academy of Scientific and Innovative Research, Ghaziabad, 201002, Uttar Pradesh, India.
International journal of biological macromolecules
|August 23, 2024
概括
这项研究开发了一种使用酸 (TA) 和聚-l-lysine (PLL) 进行生物医学植入物的混合水凝涂层. 这种新型涂层表现出强大的粘附性和有效的抗菌特性,显著减少了与植入物相关的感染.
科学领域:
- 生物材料科学 生物材料科学
- 表面化学 表面化学
- 传染病研究 传染病研究
背景情况:
- 生物医学植入物至关重要,但容易受到微生物污染,导致失败.
- 现有的水凝涂层难以平衡抗菌功效与稳定的附着性.
- 开发强大的抗感染植入物表面是一个至关重要的未满足的需求.
研究的目的:
- 创建和描述用于生物医学植入物涂层的混合水凝网络.
- 评估TA-PLL水凝的粘合,抗污染和抗菌性能.
- 评估开发的水凝涂层的生物相容性和耐用性.
主要方法:
- 使用酸 (TA) 和聚-l-lysine (PLL) 通过离子和结合制造混合水凝网络.
- 物理化学表征包括多孔性,机械测试和体外酶生物降解.
- 对粘附强度,蛋白质吸附,细菌粘附,生物膜抑制 (金黄色葡萄球菌, Pseudomonas aeruginosa) 和细胞毒性试验的评估.
主要成果:
- TA-PLL水凝表现出多孔性,良好的机械性能和酶生物降解性.
- 观察到合金 (42.5 kPa) 的强粘附性和在水中超过10天的稳定性.
- 显著减少了蛋白质吸附 (~70%),通过接触杀死机制抑制了细菌粘附,以及对S. aureus和P. aeruginosa的实质性生物膜抑制 (> 90%).
- 修改后的Ti表面保持了至少六天的生物膜抑制作用.
- 在体外细胞毒性测定证实了水凝与NIH3T3细胞的生物相容性.
结论:
- 混合TA-PLL水凝为生物医学植入物涂层提供了一个有前途的解决方案.
- 开发的水凝提供了强大的粘附性,优良的抗污染性和强大的抗感染性.
- 这项技术有可能显著缓解与植入物相关的感染,并改善患者的治疗结果.
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