长期流行病学研究中人类尿液中的物种化分析:动脉样硬化多民族研究 (MESA)
Ronald A Glabonjat1, Kathrin Schilling1, Vesna N Slavkovich1
1Columbia University, Mailman School of Public Health, Environmental Health Sciences, New York, USA.
Environmental research
|August 23, 2024
概括
这项研究开发了一种新方法来分析尿液中的物种,发现种族群体之间的暴露差异. 这些发现对于理解来说至关重要.
科学领域:
- 环境健康 环境健康
- 分析化学 分析化学
- 毒理学 毒理学 毒理学
背景情况:
- 是一种具有广泛暴露的有毒金属化物.
- 尿液中的物种化有助于理解暴露源和代谢.
- 动脉样硬化多民族研究 (MESA) 队列允许评估慢性低至中度暴露.
研究的目的:
- 介绍一种最先进的的特异化分析方法.
- 为了流行病学研究,将这种方法应用于大型队列 (MESA).
- 根据种族/种族和其他因素调查物种分泌的差异.
主要方法:
- 高性能液体色谱与诱导合等离子体质谱学 (HPLC-ICP-MS) 结合.
- 对7677个MESA点尿样品的分析.
- 包括一个氧化步骤来增强主要物种的检测.
主要成果:
- 一种可靠的方法检测11种物种,检测极限低 (0.02-0.03μg/L).
- 高精度 (98%) 和精度 (5%) 对于主要的物种.
- 亚裔美国人的度比其他群体高3-4倍.
- 排泄因种族/族裔,地点,BMI,吸烟,大米摄入量和水中的含量而异.
结论:
- 开发的HPLC-ICP-MS方法适用于大规模的流行病学研究.
- 确定了与海鲜相关的潜在代谢物.
- 在暴露中观察到显著的种族差异,突出了针对性公共卫生干预的需要.
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