相关实验视频

Updated: Jun 15, 2025

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
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A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

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基于寡核酸的CRISPR-Cas9工具箱,用于高效地设计Komagataella phaffii

Tomas Strucko1, Adrian-E Gadar-Lopez1, Frederik B Frøhling1

  • 1Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads 223, 2800 Kongens Lyngby, Denmark.

FEMS yeast research
|August 23, 2024
PubMed
概括

我们开发了一种多功能CRISPR-Cas9系统用于Komagataella phaffii (Pichia pastoris) 酵母,使得使用短DNA片段进行高效的基因编辑. 这加速了K. phaffii的生物生产工程.

关键词:
这就是Cas9的情况.KU70 KU70 KU70 KU70 KU70 KU70 KU70 KU70 KU70 KU70 KU70多重复杂的多重复杂.突变发生的突变发生.这是一种寡核化物.这是一个短暂的NHEJ.uidA标记器使用

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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

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