一个循环标记系统使克拉米多马纳斯 (Chlamydomonas reinhardtii) 中的无限系列的寡核酸导向基因编辑成为可能

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Updated: Jun 15, 2025

High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii
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High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii

Published on: December 5, 2016

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Ian L Ross1, Hong Phuong Le1, Sabar Budiman1

  • 1Institute for Molecular Bioscience (IMB), The University of Queensland, Brisbane, QLD 4072, Australia.

Plant physiology
|August 23, 2024
PubMed
概括

这项研究引入了SCREAM,这是一种针对Chlamydomonas reinhardtii的新型CRISPR基因编辑方法. 这种循环选择过程使用单个标记基因使单个细胞系中几乎无限系列的遗传修饰成为可能.

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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