Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

DNA Isolation01:24

DNA Isolation

38.5K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
38.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Novel phenotype-disease matching tool for rare genetic diseases.

Genetics in medicine : official journal of the American College of Medical Genetics·2018
Same author

Microangiopathy is associated with bone loss in female type 2 diabetes mellitus patients.

Diabetes & vascular disease research·2018
Same author

A microfluidic competitive immuno-aggregation assay for high sensitivity cell secretome detection.

Organogenesis·2018
Same author

Mussel-inspired dual-functional PEG hydrogel inducing mineralization and inhibiting infection in maxillary bone reconstruction.

Materials science & engineering. C, Materials for biological applications·2018
Same author

Evodialones A and B: Polyprenylated Acylcyclopentanone Racemates with a 3-Ethyl-1,1-diisopentyl-4-methylcyclopentane Skeleton from Evodia lepta.

Journal of natural products·2018
Same author

Application value of CT and MRI in diagnosis of primary brain lymphoma.

Oncology letters·2018
Same journal

Multi-omics analysis identifies loci associated with pyrethroid resistance across sister species in the Anopheles gambiae species complex.

BMC genomics·2026
Same journal

Comparative and population genomics analyses of eared pheasants inhabiting highly varying altitudes.

BMC genomics·2026
Same journal

Identification of differentially expressed lncRNAs in different daily weight gains of Jiangquan black pigs and functional analysis of LOC100518120.

BMC genomics·2026
Same journal

A self-attention-based deep learning model for identifying key genes in insect pupal metamorphosis.

BMC genomics·2026
Same journal

Multiple genomic approaches reveal geographic structure and local selection signals in invasive Anopheles stephensi from the Horn of Africa and Yemen.

BMC genomics·2026
Same journal

Integration of bulk RNA-seq and scRNA-seq reveals cell subsets and gene signatures associated with Glaesserella parasuis infection.

BMC genomics·2026
查看所有相关文章

相关实验视频

Updated: Jun 15, 2025

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
14:56

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Published on: May 6, 2022

4.5K

在PCA空间使用DNA甲基化进行细胞解的新方法.

Huan Xu1, Ge Zhang1,2,3, Jing Chen4,5

  • 1Division of Human Genetics, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.

BMC genomics
|August 23, 2024
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种新的细胞解方法,使用DNA甲基化数据的主要成分分析 (PCA). 该方法准确地估计了细胞组成,在区分类似细胞类型方面表现优于现有的方法.

关键词:
生物信息学是一种生物信息学.细胞解体细胞解体通过DNA甲基化.主要组件分析的主要组件分析.

更多相关视频

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

104.8K
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

9.7K

相关实验视频

Last Updated: Jun 15, 2025

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
14:56

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Published on: May 6, 2022

4.5K
DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

104.8K
Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

9.7K

科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 基于基准的细胞解对于分析复杂的生物样本至关重要.
  • 现有的方法通常依赖于探测级数据,限制了它们的范围.
  • 主要组件分析 (PCA) 为解卷提供了一个新的特征表示.

研究的目的:

  • 在DNA甲基化阵列数据上使用PCA开发和验证一种新的细胞解方法.
  • 将基于PCA的方法的性能与现有技术 (如IDOL-Ext.) 进行比较.
  • 在各种数据集中评估方法的准确性,包括临床样本.

主要方法:

  • 主要组件分析 (PCA) 空间中的特征表示.
  • 对细胞类型解的DNA甲基化阵列数据的应用.
  • 使用公共数据集的验证,包括来自质瘤患者的血液样本.

主要成果:

  • 基于PCA的方法实现了与IDOL-Ext.相比较的高准确性 (R2 0.73-0.99).
  • 改善了类似细胞类型的区分,特别是质瘤样本中的T细胞亚型 (R2 0.42-0.75与0.36-0.66).
  • 在区分某些细胞类型 (如记忆CD8T细胞) 方面仍然存在挑战.

结论:

  • 在PCA空间中细胞类型的解卷是有效的,并且得到了验证.
  • 该方法在基因组数据类型中显示了广泛的适用性.
  • 一个R包"BloodCellDecon"可供公众使用.