相关实验视频

Updated: Jun 15, 2025

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

改善了pokeweed基因组组装和早期基因表达的变化,以应对斯酸

Kyra Dougherty¹, Tanya Prashar¹, Katalin A Hudak²

¹Department of Biology, York University, 4700 Keele St, Toronto, ON, M3J 1P3, Canada.

BMC plant biology

|August 23, 2024

概括

这种草是Pokeweed.

科学领域:

植物基因组学植物基因组学
分子生物学分子生物学
生物化学生物化学

背景情况:

斯蒙酸 (JA) 是一个关键的植物激素,调节植物应激反应.
了解非模型植物 (如Phytolacca americana (pokeweed)) 中的JA通路对于更广泛的见解至关重要.
波克韦因其对环境压力因素的弹性而闻名.

研究的目的:

为了生成一个公开可用的基因组组合和pokeweed的注释.
为了研究早期基因表达的变化,以响应斯蒙酸 (JA) 治疗.
专注于参与防御机制和耐压力的基因.

主要方法:

使用PacBio Hifi进行De novo基因组组装长时间读取.
基因注释和BUSCO分析用于基因组质量评估.
在JA治疗后0-6小时时间内对pokeweed基因表达的转录组分析.

主要成果:

一个高质量的pokeweed基因组组 (NG50 ~ 13.2 Mb) 与全面的基因注释产生.
大约5,100个基因在JA治疗后表现出差异性表达,光合作用基因的显着下调和防御和激素信号基因的上调.
Pokeweed表现出二次代谢物基因的构成表达,并合成独特的RNAN-糖基酶以防御,表明长期的生存资源分配.

结论:

这项研究揭示了Pokeweed对JA的早期反应中独特的基因表达模式,突出了其防御和耐压力策略.
公共可用的pokeweed基因组为研究超越模型生物的植物防御机制提供了宝贵的资源.
这些发现为通过新的方法提高植物对环境变化的弹性提供了潜在的途径.

关键词:

美国植物 (Phytolacca americana) 是一种基因监管网络基因监管网络基因组组装组的基因组组装组雅斯蒙酸是什么意思雅斯蒙酸在Pokeweed的抗病毒蛋白质.在RNA-seqqq.压力反应应对压力.

更多相关视频

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

Published on: April 18, 2014

Detection of Histone Modifications in Plant Leaves

Detection of Histone Modifications in Plant Leaves

Published on: September 23, 2011

相关概念视频

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...

Plant Tissues

Plant Tissues

Plants are multicellular eukaryotes with tissue systems made of various cell types that carry out specific functions. Different tissues work together to perform a unique function and form an organ. Organs working together form organ systems. Vascular plants have two distinct organ systems: a shoot system and a root system. The shoot system consists of two portions: the vegetative (non-reproductive) parts of the plant, such as the leaves and the stems, and the reproductive parts of the plant,...