MdWRKY31-MdNAC7监管网络:通过调节细胞壁修饰酶MdXTH2以响应乙烯信号来调节水果软化
Jia-Hui Wang1,2, Quan Sun1, Chang-Ning Ma1
1National Research Center for Apple Engineering and Technology, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, College of Horticultural Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, China.
Plant biotechnology journal
|August 24, 2024
概括
乙烯通过抑制MdWRKY31的转录因子来触发果的软化,这种转录因子通常会抑制软化. 一个NAC TF,MdNAC7,与MdWRKY31相互作用,阻碍其功能并促进软化.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 水果软化会降低质量,并造成经济损失.
- 乙烯调节果软化,但分子机制尚不清楚.
- 了解乙烯在水果软化中的作用对于收获后管理至关重要.
研究的目的:
- 为了阐明果中以乙烯为媒介的水果软化的分子机制.
- 为了确定参与调节果坚度的关键转录因子.
- 研究乙烯信号与水果软化之间的相互作用.
主要方法:
- 转录因子MdWRKY31的鉴定和特征.
- 使用转基因方法来研究基因功能.
- 酵母单杂交,EMSA和双露西法酶测定以确认蛋白质-DNA相互作用.
- 分析了MdNAC7和MdWRKY31.31之间的蛋白质与蛋白质相互作用.
主要成果:
- MdWRKY31通过抑制MdXTH2表达来抑制乙烯诱导的软化.
- MdWRKY31通过W-box直接与MdXTH2促进体结合.
- MdNAC7促进软化,并与MdWRKY31相互作用,破坏其与MdXTH2促进体的结合.
- NAC7-WRKY31复合体调解了乙烯对XTH2表达的影响.
结论:
- MdNAC7-MdWRKY31复合体是乙烯响应信号通路的关键调节器.
- 这个复合体将乙烯信号连接到XTH2表达,促进水果软化.
- 结果提供了关于果坚度调节的见解,以及改善质量的潜在策略.
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