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异素通过调节肠上皮质P-糖蛋白 (P-gp) 表达来缓解DSS治疗的小鼠急性结肠炎

Zhenzhen Wang¹, Lanzhu Yang¹, Yun Feng²

¹College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.

DNA and cell biology

|August 24, 2024

概括

异素 (ISO) 通过上调P-糖蛋白 (P-gp) 表达来缓解炎症性肠病 (IBD). 这通过调节肠道微生物群发生,增加有益的代谢物,如短链脂肪酸 (SCFA) 和二次胆汁酸 (SBA),并恢复肠道平衡.

科学领域:

*药理学和毒理学 *药理学和毒理学
* 胃肠病学和肝病学
* 微生物学微生物学

背景情况:

* 异素 (ISO) 是一种天然的黄类化合物,具有抗炎和抗氧化特性.
*肠表皮中的P-糖蛋白 (P-gp) 与炎症性肠病 (IBD) 有关.
*肠道微生物群衍生代谢物,包括短链脂肪酸 (SCFA) 和二次胆汁酸 (SBA),影响P-gp表达和肠道炎症.

研究的目的:

* 调查ISO是否调节肠道微生物群和代谢物以影响P-gp表达并减轻IBD.
* 探索ISO在硫酸 (DSS) 诱导的大肠炎模型中的治疗潜力.
*阐明ISO影响肠道平衡的机制.

主要方法:

*用ISO.治疗小鼠的硫酸德克斯 (DSS) 诱导的大肠炎模型.
* 西方斑点分析以评估P-gp表达.
* 16S rRNA基因测序用于肠道微生物多样性分析.
* 便微生物群移植 (FMT) 从接受ISO治疗的供体到接受DSS治疗的接受者.

主要成果:

*ISO治疗减轻了DSS诱导的大肠炎症状和病理损伤.
* 在小鼠结肠组织中ISO上调的P-gp表达.
*ISO恢复了肠道微生物多样性,并丰富了SCFA生产细菌.
*ISO-FMT显著降低了结肠炎症状和增加了P-gp表达.
* 在SCFA/SBA产量和P-gp表达之间观察到正相关性.

结论:

*ISO通过增强肠表皮中的P-gp表达来缓解大肠炎.
*ISO通过调节肠道微生物群组成和代谢物产生 (SCFA和SBA) 来发挥其作用.
*ISO治疗改善了结肠上皮的完整性,并保持了肠道平衡,为IBD提供了潜在的治疗策略.

关键词:

这是一种P-glycoprotein蛋白.大肠炎症结肠炎症便微生物群移植这是一种异黄 (isoflavone).天然的黄类化合物.

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