通过固相提取和LC-HRMS在干血斑点中敏感检测低分子质量的类药物
Wei Chang1, Siyu Yan2,3, Xiya Yan2,3
1Beijing Anti-Doping Laboratory, Beijing Sport University, Beijing, People's Republic of China. nadl.changwei@bsu.edu.cn.
Analytical and bioanalytical chemistry
|August 24, 2024
概括
一种新方法增强了抗兴奋剂中低分子质量 (LMMP) 药物的干血斑 (DBS) 分析. 这种敏感的技术提高了没有二甲基硫氧化物 (DMSO) 的检测极限,有助于常规药物查.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 运动科学 运动科学 运动科学
背景情况:
- 干血斑点 (DBS) 分析在反兴奋剂中越来越受欢迎,因为样品的稳定性和易用性.
- 在DBS中的小样本体积限制了对禁止物质的敏感检测方法的应用.
- 开发高度敏感的检测方法对于使用DBS的有效反兴奋剂策略至关重要.
研究的目的:
- 在干血斑点 (DBS) 中建立低分子量 (LMMP) 药物的敏感检测方法.
- 在不使用二甲基硫氧化物 (DMSO) 的情况下,优化DBS中LMMPs的检测策略.
- 根据世界反兴奋剂机构 (WADA) 的指导方针,验证开发的常规反兴奋剂分析方法.
主要方法:
- 应用了系统优化策略来检测DBS中的LMMP.
- 该方法是在不使用DMSO的情况下开发的,以提高质谱仪的电离效率.
- 该方法根据WADA的指导方针进行了验证,包括一个后给药研究.
主要成果:
- 开发的方法实现了LMMPs的检测极限 (LOD),从0.05到3.74 ng/mL.
- 这些LOD明显低于之前报告的方法 (0.5-20 ng/mL).
- 该方法证明了在DBS中对LMMP药物的常规分析的适用性,以及同时检测LMMP和小分子药物的潜力.
结论:
- 在DBS中成功建立并验证了一种新的,敏感的LMMP检测方法.
- 与现有技术相比,该方法提供了更好的检测极限,并且避免了使用DMSO.
- 这种方法支持常规的反兴奋剂分析,并有可能在DBS中获得更广泛的药物查应用.
相关概念视频
01:33Peptide Identification Using Tandem Mass Spectrometry
6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K
01:15High-Performance Liquid Chromatography: Types of Detectors
511
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
511