通过调节miR-450a和激活PI3K/mTOR通路,CircCOCH在肝细胞癌中发挥着至关重要的作用
Weiwei Jiang1, Yan Wang2, Wanli He1
1School of Medicine, Shangqiu Institute of Technology, Shangqiu, China.
Translational oncology
|August 24, 2024
概括
循环RNAs (circRNAs) 在肝癌中起作用. 这项研究确定circCOCH在肝细胞癌 (HCC) 中高度表达,通过EGFR/PI3K通路促进瘤生长和转移.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 肝细胞癌 (HCC) 是一种具有不良预后的原发性肝癌.
- 循环RNAs (circRNAs) 在HCC发育中的功能尚未完全理解.
研究的目的:
- 调查circRNAs在HCC致癌和进展中的作用.
- 确定与HCC相关的特定circRNA并阐明它们的潜在机制.
主要方法:
- 分析与HCC相关的数据库和HCC组织和细胞系中的表达概况.
- 在体外和体内实验涉及circCOCH. knockdown的实验.
- 机制研究,以探索circCOCH,miR-450a和EGFR之间的相互作用.
主要成果:
- Hsa_circ_0031431 (circCOCH) 在HCC组织和细胞系中显著上调.
- 高 circCOCH 表达与瘤扩散和转移的增加相关.
- 对circCOCH的抑制抑制了HCC的生长.
- circCOCH通过海绵化miR-450a上调皮肤生长因子受体 (EGFR),激活PI3K/Akt通路.
- 在IGF2BP3中介于circCOCH生物发生.
结论:
- circCOCH是HCC进展的关键驱动因素,促进了扩散和转移.
- 环COCH/miR-450a/EGFR轴代表了HCC的一个新机制.
- circCOCH成为肝细胞癌的潜在治疗点.
相关概念视频
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
PI3K/mTOR/AKT Signaling Pathway
3.4K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.4K
Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
Interactions Between Signaling Pathways
6.2K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.2K
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K


