在Synechocystis sp.中,CupAR对碳获取复合体NDH-1MS中的关键蛋白CupA进行负控制. 在PCC 6803中

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Jiexi Liu¹, Fangfang Zheng¹, Min Xu¹

¹CAS Center for Excellence in Molecular Plant Sciences, Graduate Courses of the Chinese Academy of Sciences, Shanghai, PR China.

The Journal of biological chemistry

|August 24, 2024

概括

研究人员确定CupAR是蓝菌中低CO2诱导性NDH复合体 (NDH-1MS) 的新型子单元. 在高二氧化碳条件下,CupAR通过调节NDH-1MS复杂活性来负面调节CO2吸收.

科学领域:

蓝藻细菌生理学蓝藻细菌生理学
分子生物学分子生物学
光合作用研究研究光合作用.

背景情况:

低二氧化碳诱导性NDH复合体 (NDH-1MS) 对于蓝菌的二氧化碳缩机制至关重要.
对于NDH-1MS的精确组件和调节途径的理解尚不完全.

研究的目的:

确定蓝藻细菌中NDH-1MS复合体的新型成分和调节机制.
为了阐明新发现的基因sll1736 (cupAR) 与NDH-1MS活性相关的功能.

主要方法:

基因操纵以创建cupAR删除突变体 (ΔcupAR) 并研究基因共转录.
生化分析测量O2演变,CO2吸收,NDH-1活动和质子梯度.
蛋白相互作用研究 (共局部和共免疫沉) 调查CupAR在NDH-1MS复合体中的作用.

主要成果:

在高CO2 (HC) 条件下,特别是低pH条件下,ΔcupAR突变体表现出增强的生长.
在HC条件下,CupAR删除上调了O2演变,CO2吸收,NDH-1活动和质子积累.
CupAR被确定为NDH-1MS的新型子单元,与NdhD3和NdhF3相互作用,并负面调节CupA活动和CO2吸收.

结论:

库帕 (CupAR) 是NDH-1MS复合体的一个新型子单元,在高CO2下对其调节至关重要.
通过调节NDH-1MS复合体,CupAR通过对NdhR的积极调节来负面控制CupA活动和CO2吸收,这可能是通过对NdhR的积极调节.
这一发现为复杂的碳度机制提供了新的洞察力.

关键词:

碳化合物 (CO) 缩机制杯A 杯A 杯A 这就是CupAR.NDH1MSMS 没有综合囊 sp. 的情况. 在PCC 6803中.

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