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从Takifugu obscurus中建立和描述状细胞系,并在低温下对其基因表达特征进行转录组分析

Jie Wang¹, Shuang Han¹, Jingping Zhang¹

¹Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.

Fish & shellfish immunology

|August 24, 2024

概括

为了水产养殖研究,建立了一个新的Takifugu obscurus细胞系 (TOG). 这一细胞系为鱼类的免疫反应和耐寒机制提供了洞察力.

科学领域:

水产养殖是水产养殖的一种方式.
鱼类细胞生物学鱼类细胞生物学
免疫学免疫学免疫学
压力生理学压力生理学

背景情况:

在中国,Takifugu obscurus水产养殖在经济上很重要.
疾病和低温压力导致T. obscurus养殖的重大损失.
在鱼类生理学和病理学的体外研究中,teleost细胞系至关重要.

研究的目的:

为研究建立一个新的T. obscurus细胞系 (TOG).
描述TOG细胞系的特性和最佳生长条件.
研究TOG对免疫刺激和感冒压力的反应.

主要方法:

建立和长期培养T. obscurus状细胞 (TOG).
基因鉴定 (COI基因测序) 和型分析.
在冷应力下进行免疫刺激 (LPS,多 (I:C)) 和转录组分析.
权重基因同表达网络分析 (WGCW).

主要成果:

成功建立和特征TOG细胞系,稳定超过52代.
TOG细胞在刺激时表现出免疫基因上调,并在寒冷压力下在免疫和代谢途径中识别出DEG.
WGCNA揭示了关键信号通路 (PPAR,NOD类受体,Toll类受体) 和与寒冷压力和鱼类损伤相关的枢纽基因.

结论:

TOG细胞系是T. obscurus.遗传学和免疫学研究的宝贵平台.
研究结果提供了关于鱼类免疫反应和耐寒性背后的分子机制的见解.
这项研究有助于理解和减轻远距离水产养殖中寒冷压力的影响.

关键词:

细胞系细胞系细胞系吉尔·吉尔 (Gill Gill) 是一个名为吉尔的小说.免疫学免疫学免疫学低温低温的低温是什么意思塔基夫古是一个黑暗的城市.

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