Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

An encyclopedia of human enhancer-gene regulatory interactions.

Nature·2026
Same author

The Vertebrate Genomes Project Phase I: A global reference genome resource.

bioRxiv : the preprint server for biology·2026
Same author

Long-term prophylaxis in hereditary angioedema: Real-world treatment patterns and healthcare resource utilization.

The World Allergy Organization journal·2026
Same author

Diploid dual assemblies reveal the telocentric structure and extensive allelic heterogeneity of canine genomes.

NAR genomics and bioinformatics·2026
Same author

Common Coronary Artery Disease Risk Variants in Endothelial Regulatory Elements Modulate Tetraspanin 14 Expression and Notch Signaling.

Arteriosclerosis, thrombosis, and vascular biology·2026
Same author

Universal screening for hyperammonemia after lung transplantation: A retrospective cohort study of incidence and outcomes.

JHLT open·2026

相关实验视频

Updated: Jun 15, 2025

Sand Fly Phlebotomus papatasi Embryo Microinjection for CRISPR/Cas9 Mutagenesis
05:44

Sand Fly Phlebotomus papatasi Embryo Microinjection for CRISPR/Cas9 Mutagenesis

Published on: November 17, 2020

7.2K

通过超低输入的长读序列实现了改进的高质量沙飞组件.

Michelle Huang1, Sarah Kingan2, Douglas Shoue1

  • 1Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, USA.

Scientific data
|August 24, 2024
PubMed
概括

对于Phlebotomus papatasi和Lutzomyia longipalpis沙产生了新的高质量的参考基因组. 这些改进的组件将促进莱什曼病载体生物学和流行病学的研究.

更多相关视频

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen
10:28

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

Published on: July 15, 2018

9.5K
An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
12:10

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus

Published on: November 30, 2014

13.3K

相关实验视频

Last Updated: Jun 15, 2025

Sand Fly Phlebotomus papatasi Embryo Microinjection for CRISPR/Cas9 Mutagenesis
05:44

Sand Fly Phlebotomus papatasi Embryo Microinjection for CRISPR/Cas9 Mutagenesis

Published on: November 17, 2020

7.2K
Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen
10:28

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

Published on: July 15, 2018

9.5K
An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
12:10

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus

Published on: November 30, 2014

13.3K

科学领域:

  • 基因组学就是基因组学.
  • 载体生物学 载体生物学
  • 热带疾病 热带疾病

背景情况:

  • 弗莱博托米因沙传播莱什曼病,这是一个被忽视的热带疾病.
  • 以前的参考基因组是有限的,阻碍了对这些疾病载体的高分辨率基因组研究.

研究的目的:

  • 为了生成更新的,高质量的染色体水平参考基因组组合两个关键的莱什曼病载体:Phlebotomus papatasi和Lutzomyia longipalpis.
  • 为了解沙生物学和莱什曼病流行病学提供增强的基因组资源.

主要方法:

  • 使用了PacBio HiFi长读序列和Hi-C技术.
  • 采用了超低输入库准备协议.
  • 生成了P. papatasi和Lu. 的染色体水平组件. 一个长的palpis.longipalpis.

主要成果:

  • 实现了P. papatasi的351.6 Mb组装,使用111.8 Mb的N50脚手架.
  • 为Lu.实现了147.8 Mb的组装. 长鱼 (longipalpis) 的脚手架 N50 的 40.6 Mb.
  • 布斯科的评估证实了昆虫的高度完整性 (94.5%的P. papatasi,95.6%的Lu. papatasi). 长皮 (英语:longipalpis) 是指一个长皮.

结论:

  • 新的参考组件代表了与以前版本相比的显著改进.
  • 这些高质量的基因组将对未来对虫和莱什曼病的基因组研究至关重要.
  • 有助于更深入地了解载体进化和疾病传播动态.