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Updated: Jun 15, 2025

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
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A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

Published on: December 9, 2017

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来自小鼠脂肪酸合成酶的马洛尼尔/乙转移酶是一种散乱的工程工具,用于编辑多基基架

Lynn Buyachuihan1, Simon Reiners1, Yue Zhao1

  • 1Institute of Organic Chemistry and Chemical Biology, Buchmann Institute for Molecular Life Sciences, Goethe University Frankfurt, 60438, Frankfurt am Main, Germany.

Communications chemistry
|August 24, 2024
PubMed
概括

这项研究引入了一种多功能的马洛尼尔/乙转移酶 (MAT) 域,用于设计模块化聚基酸合成酶 (PKSs). 这种重编程方法可以轻松地对多基基架进行区域选择性修改,从而产生新的类型.

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Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

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Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry07:27

Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

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Malonic ester synthesis is a method to obtain α substituted carboxylic acids from ꞵ-diesters such as diethyl malonate and alkyl halides.
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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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