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来自社区废水中的多种传染病原体和抗菌素耐药基因的同时生物质度和随后的量化

Anniina Sarekoski¹, Anssi Lipponen², Anna-Maria Hokajärvi²

¹Finnish Institute for Health and Welfare, Expert Microbiology Unit, Neulaniementie 4, Kuopio FI-70701, Finland; University of Helsinki, Faculty of Veterinary Medicine, Department of Food Hygiene and Environmental Health, Agnes Sjöbergin katu 2, Helsinki FI-00014, Finland.

Environment international

|August 25, 2024

概括

基于废水的监测可以同时追踪多种传染病和抗菌素耐药性基因. 这种综合性方法通过分析废水样本中的各种病原体来加强公共卫生监测.

科学领域:

环境微生物学环境微生物学
公共卫生公共卫生
分子流行病学分子流行病学

背景情况:

基于废水的监测 (WBS) 是获得人口健康数据的宝贵工具.
目前的WBS努力往往集中在单个病原体上,限制了全面的见解.
废水含有各种各样的病原体和抗菌素耐药性基因 (ARG).

研究的目的:

开发和验证一种用于多种病原体和ARG的同时WBS的方法.
调查芬兰废水中针对性病原体和ARG的流行率和趋势.
评估样本处理方法对WBS数据准确性的影响.

主要方法:

优化了废水样本的生物质度和核酸提取.
使用定量聚合酶链反应 ((RT) -qPCR和 (HT) -qPCR) 来检测病原体和ARG.
研究了冷解和不同类型的缩物对crAssphage检测的影响.

主要成果:

同时检测了SARS-CoV-2,萨波病毒,坎比洛巴克特,沙门氏菌,致病性大肠杆菌,Cryptosporidium,Giardia以及关键的ARG.
坎比洛巴克特菌的季节性繁多与畜牧业有关,而沙门氏菌的流行程度因处理厂的大小而异.
经常检测到的ARG (blaCTX-M,blaOXA48,blaNDM,blaKPC) 与人类临床隔离物中发现的相似.
冷解废水样本显著降低了crAssphage基因拷贝数量.

结论:

同时对多种病原体和ARG进行WBS是可行的,并增强公共卫生监测能力.
废水分析为病原体循环和抗菌素耐药性趋势提供了宝贵的见解.
优化方法和了解样本处理效应对于可靠的WBS数据至关重要.

关键词:

抗微生物耐药性抗微生物耐药性多种病原体多种病原体.公共卫生公共卫生监督监督监督监督监督监督废水是废水,废水就是废水.

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