气体病毒的静电气溶到水溶收集的最佳环境采样条件
Amin Piri1, Milad Massoudifarid2, Jungho Hwang2
1Department of Mechanical Engineering, Yonsei University, Seoul 03722, Republic of Korea; Institute of Engineering Research, Yonsei University, Seoul 03722, Republic of Korea.
Journal of hazardous materials
|August 25, 2024
概括
优化静电沉 (ESP) 以检测空气中的病原体需要控制特定的环境因素. 相对湿度,取样液体温度和盐度显著影响ESP样品中的收集效率和病毒恢复.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 由于病毒爆发对健康的影响,精确检测空气传播病原体至关重要.
- 静电沉 (ESP) 是一种收集生物气溶直接作为水溶的方法.
- ESP采样器的效率受到各种操作和环境参数的影响.
研究的目的:
- 调查影响气溶到水溶ESP采样器收集效率和病毒恢复的关键参数.
- 确定最佳条件,以最大限度地提高ESP采样器在空气传播病原体检测中的性能.
主要方法:
- 评估了空气相对湿度 (RH),空气温度,采样液体类型和液体温度对ESP采样器性能的影响.
- 在使用脱离离子水时,评估病毒活力与过氧化度和空气RH相关.
主要成果:
- 发现空气温度对收集效率没有显著影响.
- 空气相对湿度 (RH),采样液体温度和盐度被确定为影响采集效率的关键因素.
- 较高的空气RH导致过氧化度增加,并且在使用脱离离子水时降低了病毒活力.
结论:
- 推ESP采样器的最佳条件包括55-65%的空气RH,37°C的空气和采样液,以及使用PBS混合物中的10-20mM亚酸作为采样液.
- 保持这些条件对于最大限度地提高采集效率和确保在采样期间病毒生存能力至关重要.
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