拉帕素的目标坐标素与黄瓜幼苗的外源性黑激素调节的低温耐受性有关
Zi-Qi Pei1, Cheng Ma1, Cui-Yun Dong1
1College of Life Sciences, Northwest Normal University, Lanzhou, 730070, China.
Plant physiology and biochemistry : PPB
|August 25, 2024
概括
黑色素 (MT) 通过调节目标拉巴胺素 (TOR) 和辅酶通路来提高黄瓜对低温 (LT) 的耐受性. 这项研究揭示了MT MT.
科学领域:
- 植物科学 植物科学
- 环境压力生理学生理学
- 生物化学 生物化学
背景情况:
- 低温 (LT) 显著影响植物生长和产量.
- 众所周知,黑素 (MT) 可以提高植物对环境压力的耐受性.
- 黄瓜 (Cucumis sativus) 容易受到LT损伤.
研究的目的:
- 研究黑激素在提高黄瓜幼苗低温耐受性的作用.
- 阐明黑色素的保护作用背后的分子机制,重点关注TOR和auxin信号通路.
主要方法:
- 在LT压力下对Comt1表达和MT含量的定量分析.
- 评估外源性MT预处理对TOR,PIN1和YUC4基因表达的影响.
- 评估MT与TOR抑制剂 (AZD-8055) 或辅酶运输抑制剂 (NPA) 联合治疗对LT应激反应的影响.
主要成果:
- LT压力增加了Comt1表达,并降低了黄瓜中的MT含量.
- 外源性MT预处理显著提高了TOR,PIN1和YUC4基因表达的调节.
- 抑制TOR或auxin运输取消了MT对LT压力的保护作用,增加了ROS水平和膜损伤.
结论:
- 黑色素通过调节TOR和auxin信号通路来增强黄瓜的低温耐受性.
- TOR和auxin信号协同作用,调解黑激素对LT引起的损伤的保护作用.
- 这些发现提供了关于植物LT耐受性中黑激素的调节网络的见解.
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