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在猪模型中移植后,来自羊水的介质干细胞减少炎症并改善肺功能

Dag Edström¹, Anna Niroomand², Martin Stenlo¹

¹Wallenberg Center for Molecular Medicine, Lund University, Lund, Sweden; Department of Clinical Sciences, Lund University, Lund, Sweden; Lund Stem Cell Center, Lund University, Lund, Sweden; Department of Cardiothoracic Anesthesia and Intensive Care, Skåne University Hospital, Lund, Sweden.

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概括

胚胎液衍生的中细胞干细胞疗法在移植模型中改善了捐赠者的肺部质量和功能. 这种肺特异性细胞治疗可能会增加供体肺的可用性,并改善移植后的结果.

科学领域:

再生医学是一种再生医学.
移植免疫学移植免疫学
肺部医学肺部医学

背景情况:

低供体肺利用率阻碍了肺移植.
传染性并发症和初级移植功能障碍 (PGD) 会导致供体肺衰退.
介质细胞干细胞在治疗肺损伤和调节炎症方面表现有前途.

研究的目的:

调查源自羊水的肺特异性介质干细胞治疗在改善供体肺质量的有效性.
在肺移植模型中评估这种细胞治疗对初级移植功能障碍和术后结果的影响.

主要方法:

有诱导急性呼吸窘迫综合征的捐赠猪接受了肺收获.
摘取的肺部被输入了ex vivo (EVLP) 或不带介质干细胞治疗.
细胞治疗包括在EVLP和移植后3次剂量,然后对接受者进行PGD评估.

主要成果:

介质干细胞治疗减少了炎症标志物,包括免疫细胞计数,组织学炎症和细胞因子水平.
接受治疗的接受者表现出改善的肺功能,由更高的PaO2/FiO2比率证明.
在接受细胞治疗的组中,初级移植功能障碍的发生率降低了.

结论:

在EVLP期间和移植后进行的肺特异性介质干细胞疗法可以改善受损的供体肺的功能.
这种基于细胞的方法有可能增加移植供体肺的可用性.
治疗也可能导致肺移植接受者更好的术后结果.

关键词:

先进的治疗性药物制品.活体肺外 perfusion 透肺外 perfusion 活体肺外 perfusion 透肺部移植肺部移植中介细胞干细胞治疗.主要的移植功能障碍.

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相关概念视频

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...