相关实验视频
Updated: Jun 20, 2026

13:36
Synthetic Spider Silk Production on a Laboratory Scale
Published on: July 18, 2012
26.7K
无化学品制造的丝纤维素微球与丝I结构
Lin Zhu1, Yunfeng Dai1, Yanfei Feng1
1State Key Laboratory of New Textile Materials and Advanced Processing Technologies, School of Textile Science and Engineering, Wuhan Textile University, Wuhan 430200, China.
International journal of biological macromolecules
|August 25, 2024
概括
研究人员开发了一种绿色方法,使用静电喷雾和冷制造丝纤维素 (SF) 微球. 这些稳定,可降解的SF微球显示出药物输送和细胞培养应用的前景.
科学领域:
- 生物材料科学 生物材料科学
- 生物技术是生物技术.
- 材料化学 材料化学
背景情况:
- 丝纤维素 (SF) 微球对生物医学用途如药物输送和组织工程具有前景.
- 一个主要的挑战是为安全的生物医学应用准备SF微球的化学独立制备.
研究的目的:
- 开发一种绿色,化学独立的方法来制备丝纤维素微球.
- 描述由此产生的SF微球的稳定性,降解性和生物医学潜力.
主要方法:
- 利用静电喷射到液中,形成SF微球.
- 采用化方法将SF结晶成丝状I型晶体,产生不溶于水的微球.
- 评估了微球在水中的稳定性,PBS中的降解以及细胞培养和药物释放中的性能.
主要成果:
- 产生的SF微球具有可调节的大小和丝I晶体结构.
- 微球在水中表现出高稳定性 (长达30天) 和在生理条件下快速降解 (14天内77%).
- 成功支持哺乳动物细胞生长,并在10天内持续释放多克索鲁比.
结论:
- 为SF微球制备建立了一种绿色,无溶剂和无交叉连接剂的方法.
- 丝I SF微球具有出色的稳定性,可调节的降解性和生物相容性.
- 这些SF微球具有先进的生物医学应用的巨大潜力,包括药物输送和再生医学.
相关概念视频
Protein and Protein Structure
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme can...
A protein's shape is critical to its function. For example, an enzyme can...
Globular and Fibrous Proteins
Many proteins can be classified into two distinct subtypes - globular or fibrous. These two types differ in their shapes and solubilities.
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
The Structure of Intermediate Filaments
The intermediate filaments are one of three widely studied cytoskeletal filaments. They are so named as their diameter (10 nm) is in between that of microfilaments (7 nm) and the microtubules (25 nm). These filaments are highly stable and can remain intact when exposed to high salt concentrations and detergents. These filaments are responsible for providing stability and mechanical support to the cells. They also help in cell adhesion and maintaining tissue integrity.
Intermediate filaments...
Intermediate filaments...
Formation of Intermediate Filaments
Intermediate filaments are cytoskeletal proteins with higher tensile strength and flexibility than microfilaments and microtubules. Unlike the other two cytoskeletal proteins, intermediate filament formation lacks the enzymatic activity to hydrolyze nucleotides like ATP and GTP to generate energy for polymerization. Therefore, the formation of intermediate filaments is multistep self-assembly. The involvement of any accessory proteins in intermediate filament formation has not yet been reported.
Types of Step-Growth Polymers: Polyesters
The introduction of polyesters has brought major development to the textile industry. The wrinkle-free behavior of polyester blends has eliminated the need for starching and ironing clothes.
Polyesters are commonly prepared from terephthalic acid and ethylene glycol; the crude product is known as poly(ethylene terephthalate) or PET. However, polyesters are synthesized industrially by transesterification of dimethyl terephthalate with ethylene glycol at 150 °C. The two reactants and the polymer...
Polyesters are commonly prepared from terephthalic acid and ethylene glycol; the crude product is known as poly(ethylene terephthalate) or PET. However, polyesters are synthesized industrially by transesterification of dimethyl terephthalate with ethylene glycol at 150 °C. The two reactants and the polymer...
Fibrous Proteins
Fibrous proteins are either long and narrow proteins or assemble to form long and thin structures. They contain repetitive units and usually consist of either alpha helices or beta sheets and, in rare cases, a mix of both. The amino acids in the primary structure often consist of repeating amino acid sequences. The role of fibrous proteins is primarily structural. Many are located in the extracellular matrix and are present in connective tissues to impart strength and joint mobility. They are...

