对单分子免疫检测的审查:非芯片和芯片上的检测
1State Key Laboratory of Biochemical Engineering, PLA Key Laboratory of Biopharmaceutical Production & Formulation Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China; Key Laboratory of Biopharmaceutical Preparation and Delivery, Chinese Academy of Sciences, Beijing, 100190, China.
Analytica chimica acta
|August 25, 2024
概括
单分子免疫试验 (SMIs) 为检测低丰度蛋白质生物标志物提供了更高的灵敏度,从而促进了早期诊断. 本综述探讨了SMI技术,应用和更广泛实施的挑战.
科学领域:
- 免疫学和诊断 免疫学和诊断
- 生物标志物检测检测 生物标志物检测
- 分子检测试验 分子检测试验
背景情况:
- 免疫学中对敏感免疫测试的需求日益增加,用于检测低丰度蛋白质生物标志物.
- 基因检测的快速进步需要提高诊断灵敏度.
- 单分子免疫试验 (SMIs) 的出现作为一个关键的创新.
研究的目的:
- 审查单分子免疫试验 (SMIs) 的发展和技术途径.
- 评估和比较非芯片和芯片上的SMI方法.
- 讨论SMI的诊断意义,应用和未来方向.
主要方法:
- 审查关于免疫测试开发和SMI技术的现有文献.
- 详细讨论和比较非芯片 (没有微流体芯片) 和芯片 (有微流体芯片) 上的SMI路径.
- 分析SMI在实验室和临床环境中的应用,以及商业进展.
主要成果:
- 中小微量检测代表了免疫测试灵敏度的显著进步,使得以前无法检测的生物标志物的检测成为可能.
- 非芯片和芯片上的SMI方法都显示出明显的优势和应用.
- 中小企业在疾病诊断方面越来越重要,并在商业化方面取得了进展.
结论:
- 单分子免疫试验正在开启敏感诊断的新时代.
- 尽管有潜力,但实际应用的挑战仍然存在,特别是在资源有限的地区.
- 进一步创新和解决实施障碍对于广泛采用SMI至关重要.
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