一个保存的转录因子的代码支着真菌毒性
Jiyun Yang1, Bing Li2, Yu-Ting Pan1
1Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu, 210037, China.
BMC biology
|August 25, 2024
概括
一项研究揭示了Collettotrichum gloeosporioides如何应对细胞壁压力. 蛋白质CgMkk1将CgCrzA移动到核中,激活胺合成酶基因,这对于真菌生长和致病性至关重要.
科学领域:
- 菌类学 菌类学是指菌类学.
- 植物病理学 植物病理学
- 分子生物学分子生物学
背景情况:
- 细胞壁完整性 (CWI) 对真菌的生存,生长和发病至关重要.
- 白 (CFW) 是一种抑制真菌生长的压力剂,但其对植物病原菌的影响尚未完全理解.
研究的目的:
- 研究Collettotrichum gloeosporioides对CFW诱导的细胞壁应激反应背后的分子机制.
- 确定参与C. gloeosporioides适应细胞壁干扰物的关键蛋白质和途径.
主要方法:
- 研究了对CFW的反应中转录因子CgCrzA的转位.
- 利用蛋白相互作用研究来确定CgMkk1作为CgCrzA核进口的调节者.
- 采用染色体免疫沉测序 (ChIP-seq) 来识别CgCrzA.的下游标.
主要成果:
- CFW诱导了CgCrzA从细胞质到细胞核的转移.
- CgMkk1,一个基因激活蛋白激酶,通过酸化在Ser280.0.中介CgCrzA核进口通过酸化.
- ChIP-seq确定了编码奇合成酶的CgCHS5和CgCHS6,作为CgCrzA.A.的直接目标.
结论:
- 阐明了一种涉及CgMkk1,CgCrzA和基合成酶 (CgCHS5/6) 的新型调节途径.
- 这一途径对于C. gloeosporioides对CFW等细胞壁抑制剂的反应至关重要.
- 这些发现增强了对真菌病原性和细胞壁动态的理解.
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