作为人类基因组中cis调节元件的关键组成部分的G四重复
Rongxin Zhang1,2, Yuqi Wang2, Cheng Wang3,4
1Laboratoire d'Optique et Biosciences (LOB), Ecole Polytechnique, CNRS, INSERM, Institut Polytechnique de Paris, 91120, Palaiseau, France.
BMC biology
|August 25, 2024
概括
G四重复体 (G4s) 是人类 cis调节元件 (CREs) 的组成部分,影响其局部化和激活. 这些DNA结构与调节潜力有关,并且在癌症中被激活,这表明G4s是关键的基因组调节者.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 控制基因表达的Cis调节元件 (CREs).
- G四复合体 (G4s) 是非正规的DNA结构,具有潜在的调节作用.
- 需要系统地调查G4和非推动者CREs之间的相互作用.
研究的目的:
- 调查G-四重复合体 (G4s) 和人类候选 cis调节元件 (cCREs) 之间的关联.
- 了解G4s在CREs的功能和调节中的作用.
主要方法:
- 分析DNA元素百科全书 (ENCODE) 的数据.
- 研究了G4s与CREs和表观遗传标记 (CTCF,H3K4me3,H3K27ac) 的同时发生情况.
- 评估了G4s和CREs中的遗传变异的进化保护和调节潜力.
主要成果:
- G4s富含大多数人类的cCREs,特别是促销器类元素.
- G4s中的遗传变异显示出更高的调节潜力和有害影响.
- G4的存在与有利于CRE激活的染色质环境相关.
- 与G4相关的cCREs在各种癌症中被广泛激活.
结论:
- G四重复体 (G4s) 是人类 cis调节元件 (CREs) 的组成部分.
- G4序列影响CRE本地化,G4结构有助于CRE激活.
- 应该承认G4s是人类基因组中关键的调节元素.
相关概念视频
Cis-regulatory Sequences
9.8K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.8K
Cooperative Binding of Transcription Regulators
6.4K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.4K
Combinatorial Gene Control
8.3K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.3K
Co-activators and Co-repressors
7.3K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.3K
Covalently Linked Protein Regulators
6.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
6.8K
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K


