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在饥饿期间,DoSPX1和DoMYB37调节了Dendrobium officinale中DoCSLA6的表达

ZhiYuan Feng¹, YaWen Li¹, SiXue Zhang¹

¹School of Life Sciences, Anhui Agricultural University, Hefei, Anhui, 230036, People's Republic of China.

BMC plant biology

|August 25, 2024

概括

在Dendrobium officinale中缺乏可以增加多糖的积累. DoSPX1-DoMYB37-DoCSLA6通路调节了这种反应,改善了植物对低条件的耐受性.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

丹德罗毕 (Dendrobium officinale) 的生长对营养物质的可用性,特别是的可用性很敏感.
众所周知,缺乏会增加D. officinale.中的多糖积累.
将低与多糖化合物合成联系起来的分子机制在很大程度上是未知的.

研究的目的:

在缺乏的条件下,阐明调节D. officinale中多糖积累的分子途径.
为了确定关键的基因和蛋白质相互作用涉及低耐受性.

主要方法:

酵母一混合 (Y1H) 和电泳运动转移试验 (EMSA) 用于确定基因促进体结合.
在D. officinale原型体中,基因的过度表达是暂时的.
测量植物酸盐含量和基因表达水平.

主要成果:

DoSPX1被确定为一种减少植物酸盐积累的因素,并提高酸盐饥饿诱导 (PSI) 基因的调节.
已经证明DoMYB37可以结合DoCSLA6的促进子,这是聚糖合物的关键基因.
在D. officinale原型体中,DoSPX1和DoMYB37的同时表达导致酸盐含量降低,DoCSLA6的表达增加.

结论:

阐明了涉及DoSPX1,DoMYB37和DoCSLA6的信号通路.
这一途径为D. officinale在饥饿下增加多糖积累提供了分子基础.
这些发现为了解和潜在地操纵药用植物中的多糖含量提供了理论基础.

关键词:

丹德罗比亚公办植物 (Dendrobium officinale) 是一种植物.葡萄康曼南 (Glucomannan) 是一种葡萄糖.MYB家族是MYB家族的一个组成部分.的饥饿是因为的饥饿.

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