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Updated: Jun 15, 2025

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Published on: May 25, 2018

在活细胞中运行的RNA激活CRISPR/Cas12a纳米机器人

Aijiao Yuan^1,2, Rui Sha^1,2, Wenjing Xie^1,2

¹State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.

Journal of the American Chemical Society

|August 26, 2024

概括

RNA可以激活CRISPR/Cas12a系统,使新的诊断工具成为可能. 这种RNA激活的CRISPR/Cas12a系统被用来创建CRISPR纳米机器人,用于在活细胞中检测敏感的微RNA.

科学领域:

分子生物学
生物技术
纳米技术

背景情况:

CRISPR/Cas12a系统表现出 cis-和 trans-cleavage 活动,主要由 DNA 激活.
RNA在激活CRISPR/Cas12a及其跨裂变活性中的作用尚不清楚.
了解RNA激活对于扩大CRISPR/Cas12a应用至关重要.

研究的目的:

研究CRISPR/Cas12a系统的RNA激活.
描述由RNA触发的跨裂变活动.
开发RNA激活的CRISPR纳米机器人用于细胞成像.

主要方法:

在金纳米颗粒上组装CRISPR/Cas12a核蛋白 (RNP) 和核酸基质.
使用微RNA结合来激活CRISPR纳米机器人和它们的跨裂变功能.
活细胞中微RNA的实时光成像.

主要成果:

RNA成功地激活了CRISPR/Cas12a系统,触发了跨裂变活动.
已激活的CRISPR/Cas12a RNP更喜欢使用更长的序列.
CRISPR纳米机器人通过重复的跨裂变显示了放大光信号.
在活细胞中实现了敏感的实时检测和特定微RNA的成像.

结论:

RNA可以激活CRISPR/Cas12a系统,扩大它们的激活机制.
激活CRISPR纳米机器人的开发使敏感的细胞内微RNA检测成为可能.
这项技术在监测和调节活细胞中的生物功能方面具有重大潜力.

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