从Hedychium spicatum (生科) 的基因组组合草案和基因型数据中鉴定SSR标记物的特征
1Tropical Ecology and Evolution (TrEE) Lab, Department of Biological Sciences, Indian Institute of Science Education and Research Bhopal (IISER Bhopal), Madhya Pradesh 462066, India.
Data in brief
|August 26, 2024
概括
我们为药用草药Hedychium spicatum开发了新的简单序列重复 (SSR) 标记. 这些具有成本效益的标记物对Hedychium物种的遗传研究有用.
科学领域:
- 基因组学就是基因组学.
- 植物科学 植物科学
- 分子生物学分子生物学
背景情况:
- 紫杉科 (Zingiberaceae) 家族包括许多具有药用价值的热带植物.
- 刺 (Hedychium spicatum) 是这个家族中的一个重要物种,因其药用特性而被利用.
研究的目的:
- 为了对Hedychium spicatum进行基因组组装.
- 识别和表征简单序列重复 (SSR) 标记器用于基因分析.
- 评估这些标记物在相关的Hedychium物种中的有用性.
主要方法:
- 基因组组合的Hedychium spicatum. 这种植物.
- 在核,质体和线粒体基因组中识别了60,695个SSR.
- 设计和测试了14851个SSR启动器对.
- 20个验证的SSR标志物的特征,其中11个用于基因型定型,99个加入和四个同源物种.
主要成果:
- 成功生成了一个连接层次的基因组组.
- 20个SSR标记物的特征,显示放大成功和模式保留.
- 11个SSR标记在四种相关的Hedychium物种中表现出多态性和跨物种放大.
- 每个位点的等位基多样性在5到25之间.
结论:
- 开发的SSR标记物对于Hedychium的遗传研究是有效和成本效益的.
- 这些标记物在种群遗传学,DNA指纹和Hedychium物种多样性评估方面具有广泛的实用性.
- 这些发现促进了对Hedychium的遗传多样性和种群结构的进一步研究.
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