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相关概念视频

Overview of Metabolism01:40

Overview of Metabolism

29.7K
Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
29.7K
Fates of Pyruvate01:20

Fates of Pyruvate

8.4K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
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Dietary Connections01:23

Dietary Connections

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In biological systems, most metabolic pathways are interconnected. The cellular respiration processes that convert glucose to ATP—such as glycolysis, pyruvate oxidation, and the citric acid cycle—tie into those that break down other organic compounds. As a result, various foods—from apples to cheese to guacamole—end up as ATP. In addition to carbohydrates, food also contains proteins and lipids—such as cholesterol and fats. All of these organic compounds are used...
50.1K
Peroxisomes and Mitochondria01:30

Peroxisomes and Mitochondria

86.9K
Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
86.9K
Fermentation01:29

Fermentation

113.5K
Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
113.5K
Role of Reduced Coenzymes NADH and FADH₂01:29

Role of Reduced Coenzymes NADH and FADH₂

11.3K
The energy released from the breakdown of the chemical bonds within nutrients can be stored either through the reduction of electron carriers or in the bonds of adenosine triphosphate (ATP). In living systems, a small class of compounds functions as mobile electron carriers, molecules that bind to and shuttle high-energy electrons between compounds in pathways. The principal electron carriers that will be considered originate from the B vitamin group and are derivatives of nucleotides; they are...
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Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
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Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

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组装细菌拼图:将功能拼接成微生物通路.

Henri C Chung1,2, Iddo Friedberg2, Yana Bromberg3,4

  • 1Program in Bioinformatics and Computational Biology, Iowa State University, Ames, IA 50011 , USA.

NAR genomics and bioinformatics
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概括
此摘要是机器生成的。

这项研究引入了一种新的方法,使用并发分析从元基因组数据中识别微生物途径,改善功能洞察力和预测未知的生物功能.

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Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
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Functional Complementation Analysis FCA: A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways
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Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
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Functional Complementation Analysis FCA: A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways
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科学领域:

  • 微生物学 微生物学
  • 生物信息学是一种生物信息学.
  • 系统生物学 系统生物学

背景情况:

  • 功能性元基因组学提供了对微生物群落的洞察力,但缺乏优质的注释.
  • 由于功能数据有限,发现生物途径具有挑战性.

研究的目的:

  • 开发一种基于共发生的方法,从基因组和元基因组数据中发现微生物途径.
  • 改进酶之间的功能关系的识别,并预测新的途径.

主要方法:

  • 利用了家族遗传特征来对预测的微生物蛋白质功能进行基于共发生的分析.
  • 用最少的数据进行路径识别的优化配置设计.
  • 聚类酶对成多酶途径,并与KEGG数据库进行验证.

主要成果:

  • 在识别功能关系方面,基因组概况方法的表现优于基于同质性的方法.
  • 成功预测了新出现的生物化学途径,包括那些功能不明的生物化学途径.
  • 证明了一种用于预测海洋微生物群中的细菌间蛋白相互作用的新型扩展.

结论:

  • 开发的方法增强了整个元基因组内的潜在功能能力的识别.
  • 建立了发现以前未知的和抽象的生物功能作为离散途径的基础.