马类三体病:分子检测,血液学和氧化应激分析
Mostafa Shoraba1, Salma A Shoulah1, Faysal Arnaout1
1Department of Animal Medicine (Infectious Diseases) Faculty of Veterinary Medicine Benha University, Toukh 13736, Egypt.
Veterinary medicine international
|August 26, 2024
概括
埃及马的苏拉感染显著改变了血液参数,增加了氧化应激. 分子分析证实了Trypanosoma evansi的存在,并确定了它与印度分离品的遗传关系.
科学领域:
- 兽医寄生虫学 兽医寄生虫学
- 分子生物学分子生物学
- 马的健康 马的健康
背景情况:
- 由Trypanosoma evansi引起的Surra,影响着全球的马类行业.
- 关于埃及马中的T. evansi的遗传特征存在有限的数据.
研究的目的:
- 在埃及马匹中进行T. evansi的分子表征.
- 评估与T. evansi感染相关的血液学变化和氧化应激标志物.
主要方法:
- 针对RoTat 1.2 VSG基因进行的PCR用于T. evansi检测.
- 血液学参数 (RBC,HCT,Hb,MCHC,MCH,MCV) 和氧化应激标志物 (GSH,SOD,CAT,TAC) 的评估.
- 对T. evansi菌株的遗传学分析.
主要成果:
- 所有检查的马通过PCR检测出T. evansi阳性.
- 受感染的马体显示红血球,红血球,红血球和红血球减少,MCHC,MCH和MCV增加.
- 在受感染的马中观察到GSH,SOD,CAT和TAC的水平降低,同时出现单细胞减小,乙减小和淋巴细胞减小.
- 遗传学分析表明,埃及T. evansi菌株与印度孤立菌株密切相关.
结论:
- 马中的T. evansi感染会导致显著的血液学变化和氧化应激.
- 这项研究为埃及马中的T. evansi提供了第一个分子特征.
- 这些发现突出了埃及和印度T. evansi菌株的遗传关系.
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