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Updated: Jun 15, 2025

Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
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Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses

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对逆转录聚合酶连锁反应进行比较分析,用于艾奇病毒检测

Mi-Gyeong Kim1, Boeun Yoo1, A Young Min1

  • 1Food Microbiology Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, 187 Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do 28159 Republic of Korea.

Food science and biotechnology
|August 26, 2024
PubMed
概括

艾滋病毒-A (AiV-A) 在全球范围内引起胃肠炎. 这项研究开发了一种有效的聚合酶连锁反应 (PCR) 方法,用于检测受污染的环境和食品中的AiV.

关键词:
一个艾奇病毒病毒.传统的RT-PCR可以使用.通过食物传播的病毒人类胃肠炎的人类胃肠炎实时RT-PCR实时RT-PCR的时间

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Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction08:36

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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