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Updated: Jun 15, 2025

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

鉴定活体内化心脏特异性RNA吸收体的内化

Chandan Narayan¹, Li-Hsien Lin¹, Maya N Barros¹

¹Department of Internal Medicine, University of Iowa, Iowa City, IA, USA.

bioRxiv : the preprint server for biology

|August 26, 2024

概括

研究人员开发了用于向心脏药物输送的新型吸收体. 这些体选择性地结合并进入心脏细胞,显示出治疗心脏病和改善心脏成像的潜力.

科学领域:

生物技术是生物技术.
分子生物学分子生物学
心血管研究研究心血管研究

背景情况:

针对特定组织的向治疗对治疗疾病,特别是心脏病的治疗至关重要.
开发特定于心脏的配体对于有效的针对心脏的治疗是必不可少的.

研究的目的:

开发和评估对心室心肌细胞具有高特异性的新型体.
评估这些阿普坦体在向药物输送和心脏成像方面的潜力.

主要方法:

使用ex vivo和in vivo SELEX (通过指数式丰富对接体的系统进化) 来丰富体.
选了2'-改性体的图书馆,以检测心室心肌细胞特异性.
在ex vivo和in vivo小鼠模型中评估了亚的结合和内部化.

主要成果:

在非心脏组织 (如肝脏和骨肌肉) 上,SELEX产生了具有显著心脏特异性的体.
性亚体CA1在体内表现出有选择性的向和输送光体到小鼠腹腔心肌细胞.
CA1主要内化到心肌细胞中,而CA41主要与细胞表面结合.

结论:

在心脏病中,aptamers CA1 和 CA41 显示出针对性药物输送的前景.
这些体是适合用于心脏成像应用的候选者.
这一发现支持了aptamer技术在心血管医学中的潜力.

关键词:

这就是SELEXEX.一个aptamer的应用程序.针对心肌细胞的向带.

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相关概念视频

In-situ Hybridization

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...