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Updated: Jun 15, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
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在单个步骤中使用工程 HUH-Tags 进行序列定向的共价蛋白-RNA 连接

Adam T Smiley1, Natalia Babilonia-Díaz1, August J Krueger1

  • 1University of Minnesota, Department of Biochemistry, Molecular Biology, and Biophysics.

bioRxiv : the preprint server for biology
|August 26, 2024
PubMed
概括

工程HUH标签现在可以与RNA形成特定的共价键,扩大它们在生物技术中的使用范围. 这一进步为新型应用提供了精确的蛋白质-RNA生物结合.

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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