在行为哺乳动物的多个神经元类中成像高频电压动态
Simon Haziza1,2, Radosław Chrapkiewicz1,2, Yanping Zhang1,2,3
1James H. Clark Center, Stanford University, Stanford, CA 94305, USA.
bioRxiv : the preprint server for biology
|August 26, 2024
概括
新的TEMPO电压传感技术实现了高灵敏度,可追踪高达100赫兹的神经振荡. 这些工具使得能够详细研究神经动力学和神经相互作用,无论是健康的还是患病的大脑.
科学领域:
- 神经科学是一个神经科学.
- 生物技术是生物技术.
- 光学成像技术的成像
背景情况:
- 遗传编码的电压指示器对于监测神经活动至关重要.
- 现有的电压成像仪器对高频神经振荡缺乏灵敏度.
- 追踪神经振荡对于理解大脑功能至关重要.
研究的目的:
- 引入两种新的TEMPO电压传感技术,用于敏感的神经振荡检测.
- 为了在目标细胞群中实现高频电压成像.
- 在体内研究神经动力学和细胞类型相互作用.
主要方法:
- 开发和应用光纤TEMPO用于敏感光度测量.
- 使用TEMPO介光镜进行广场电压成像.
- 在自由移动和头部固定的小鼠中记录神经振荡高达~100 Hz.
主要成果:
- 光纤TEMPO的灵敏度增加了10倍,可以进行长达1小时的录音.
- 在海马波中发现了theta和gamma振荡的交叉频率合.
- 波测距仪揭示了感官唤起的刺激-抑制相互作用和视觉皮层中的移动波.
结论:
- 时间技术显著提升了图像神经电压振荡的能力.
- 这些工具为神经动力学提供了新的见解,包括以前未报告的波传播方向.
- 预计将广泛应用于在各种神经疾病中探测大脑振荡.
相关概念视频
Action Potentials
Overview
Action Potential
Neurons communicate by firing action potentials—the electrochemical signal that is propagated along the axon. The signal results in the release of neurotransmitters at axon terminals, thereby transmitting information to the nervous system. An action potential is a specific "all-or-none" change in membrane potential that results in a rapid spike in voltage.
Membrane potential in neurons
Neurons typically have a resting membrane potential of about -70 millivolts (mV). When they receive...
Membrane potential in neurons
Neurons typically have a resting membrane potential of about -70 millivolts (mV). When they receive...
Functions of the Nervous System
The nervous system is responsible for coordinating and regulating the body's functions. It functions through three main processes: sensory, integrative, and motor processes. Sensory function involves the detection and transmission of information about internal and external stimuli from sensory receptors to the CNS. The CNS processes this information through an integrative function, where it interprets and makes decisions based on the incoming sensory information. Finally, the motor function...
Action Potential: Phases of Stimulation
The action potential is a complex electrical event that occurs in excitable cells, such as neurons and muscle cells. It consists of several distinct phases, each with specific characteristics.
Resting Phase:
In this phase, the cell's membrane is at its resting potential, typically around -70 millivolts (mV) for neurons. Inside the cell, there is a higher concentration of potassium ions (K+) and a lower concentration of sodium ions (Na+). Voltage-gated sodium channels are closed, and...
Resting Phase:
In this phase, the cell's membrane is at its resting potential, typically around -70 millivolts (mV) for neurons. Inside the cell, there is a higher concentration of potassium ions (K+) and a lower concentration of sodium ions (Na+). Voltage-gated sodium channels are closed, and...
Propagation of Action Potentials
The propagation of an action potential refers to the process by which a nerve impulse, or "action potential," travels along a neuron.
Neurons (nerve cells) have a resting membrane potential, with a slightly negative charge inside compared to outside. This is maintained by ion channels, such as sodium (Na+) and potassium (K+) channels, which control the flow of ions. When a stimulus, like a touch or a signal from another neuron, triggers the neuron, sodium channels open, allowing sodium ions to...
Neurons (nerve cells) have a resting membrane potential, with a slightly negative charge inside compared to outside. This is maintained by ion channels, such as sodium (Na+) and potassium (K+) channels, which control the flow of ions. When a stimulus, like a touch or a signal from another neuron, triggers the neuron, sodium channels open, allowing sodium ions to...
Neuronal Communication
Neurons, the fundamental units of the brain and nervous system, communicate through complex electrochemical signals that underpin all cognitive and bodily functions. This communication is primarily facilitated by a process involving the generation and propagation of an action potential along the axon of the neuron. When the internal electrical charge of a neuron surpasses a certain threshold, an action potential is triggered. This rapid change in voltage travels swiftly along the axon to the...


