Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Phase II Reactions: Methylation Reactions01:17

Phase II Reactions: Methylation Reactions

161
Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...
161
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

11.9K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
11.9K
Disubstituted Cyclohexanes: cis-trans Isomerism02:37

Disubstituted Cyclohexanes: cis-trans Isomerism

11.8K
Depending upon the different spatial orientation of the substituents, the disubstituted cycloalkanes exhibit two types of stereoisomers. The cis isomers have the substituents on the same side of the ring, whereas the trans isomers have the substituents on the opposite sides. These stereoisomers exhibit different physical properties and cannot be interconverted without breaking the carbon-carbon bonds.
In cyclohexane, the substituents can occupy different positions generating distinct isomers....
11.8K
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

9.9K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
9.9K
tRNA Activation02:26

tRNA Activation

19.1K
Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...
19.1K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

24.0K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
24.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Hit-to-Lead Optimization of Energy-Coupling Factor (ECF) Transporter Inhibitors as Novel Antibiotic.

Journal of medicinal chemistry·2026
Same author

Aggregicyclins Shed Light on Type II Polyketide Biosynthesis in <i>Myxococcota</i>.

JACS Au·2026
Same author

Structural and biochemical characterization of yeast Tcd enzymes installing the post-transcriptional modification ct6A in tRNA.

Nucleic acids research·2026
Same author

Structural basis of membrane potential coupled vectorial CO₂ hydration by the DAB2 complex in chemolithoautotrophs.

Nature communications·2026
Same author

Integrating chemical, genetic, and feasibility assessments for anti-tubercular target validation.

EMBO molecular medicine·2026
Same author

Genome Mining Guided Identification of the Metallophore Delftichelin A from <i>Delftia deserti</i>.

Journal of natural products·2026

相关实验视频

Updated: Jun 15, 2025

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
12:07

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Published on: November 22, 2014

14.0K

异功能但结构上不同的甲基转移酶用于迪提奥皮罗龙的多样化.

Li Su, Eva M Huber1, Margaretha Westphalen2

  • 1Technical University of Munich, TUM School of Natural Sciences, Department of Bioscience, Center for Protein Assemblies, 85748, Garching, Germany.

Angewandte Chemie (International ed. in English)
|August 26, 2024
PubMed
概括

研究人员确定了XrdM酶,该酶负责细菌中甲基化纳丁 (XRD). 这一发现揭示了dithiolopyrrolone (DTP) 生物合成,并揭示了融合酶的进化.

关键词:
生物合成生物合成迪西奥洛皮罗隆天然产品酶催化酶的催化作用在NRPS后的甲基化后.结构生物学结构生物学

更多相关视频

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling
08:34

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

Published on: December 18, 2017

6.6K
Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
08:48

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Published on: November 29, 2014

13.9K

相关实验视频

Last Updated: Jun 15, 2025

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
12:07

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Published on: November 22, 2014

14.0K
An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling
08:34

An Engineered Split-TET2 Enzyme for Chemical-inducible DNA Hydroxymethylation and Epigenetic Remodeling

Published on: December 18, 2017

6.6K
Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
08:48

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Published on: November 29, 2014

13.9K

科学领域:

  • 生物化学 生化学
  • 分子生物学分子生物学
  • 自然产品生物合成 自然产品生物合成

背景情况:

  • 迪提奥皮罗隆 (DTP) 天然产品具有显著的抗菌,抗真菌和抗癌特性.
  • 对于微调生物活性而言,DTP核心的胺N-甲基化是至关重要的,但负责的酶往往没有特征.
  • 谢诺哈布丁 (XRD) 是一种DTP天然产品,具有潜在的治疗应用.

研究的目的:

  • 确定负责Xenorhabdus doucetiae中的Xenorhabdin (XRD) 胺基N-甲基化酶.
  • 研究XrdM与其他DTP相关的甲基转移酶之间的结构和功能关系.
  • 扩大对DTP生物合成中的非核糖体后合成酶 (NRPS) 胺基甲基化的理解.

主要方法:

  • 基因鉴定和XrdM甲基转移酶的特征.
  • 进行X射线晶体学以确定酶结构.
  • 酶活性测定和位点定向突变发生,以探测功能.
  • 生物信息分析和比较建模.

主要成果:

  • 胺基甲基转移酶XrdM被确定为负责Xenorhabdus doucetiae中的Xenorhabdin (XRD) 甲基化.
  • 在功能上,XrdM与DtpM (参与硫素生物合成) 类似,但具有与X射线无关的X射线结构.
  • 这项研究揭示了两个结构上不同的酶的融合进化,它们催化了相同的甲基化反应.

结论:

  • XrdM是一种参与DTP生物合成的新型酶,扩展了已知的NRPS后修饰机制.
  • 这些发现突显了酶功能的融合进化,相同的生物化学反应具有不同的结构解决方案.
  • 这项研究提供了对自然产品生物合成和酶进化的多样性的见解.