使用空心纤维膜从发酵中持续液体液体提取中链脂肪酸
Kayode J Taiwo1, Hubert Nguyen1, Joseph G Usack2
1Department of Food Science and Technology, University of Georgia.
Journal of visualized experiments : JoVE
|August 26, 2024
概括
中链脂肪酸 (MCFA) 的可持续生产至关重要. 这项研究表明,液体-液体提取 (LLE) 可以有效地从发酵中去除MCFA,从而使产品更纯净,避免传统来源的生态损害.
科学领域:
- 生物技术是生物技术.
- 化学工程是化学工程的重要组成部分.
- 可持续化学 可持续化学
背景情况:
- 中链脂肪酸 (MCFA) 是有价值的平台化学物质,具有多种工业应用.
- 目前的生产依赖于棕油和子油,造成了严重的生态破坏.
- 使用低价值原料的可持续生物生产方法正在开发中.
研究的目的:
- 为了证明液体-液体提取 (LLE) 的应用,以持续去除MCFA.
- 通过发酵提高MCFA生产的可持续性和效率.
- 为了促进用于下游加工的纯MCFA的回收.
主要方法:
- 使用微生物链延长 (CE) 作为参考发酵系统.
- 使用空心纤维膜的液体-液体提取 (LLE).
- 在矿物油中使用3% (w/v) 的三聚酸氧化物作为去除MCFA的提取剂系统.
主要成果:
- 从酸 (C5) 到酸 (C8) 的MCFA从发酵中被选择性地去除.
- 持续的现场提取防止了产品的抑制,并促进了恢复.
- 在性剥离溶液中将MCFA缩到高位数.
结论:
- 液体-液体提取是一种可靠的方法,用于从发酵中连续去除MCFA.
- 这种方法通过有效的回收和净化来支持可持续的MCFA生产.
- 开发的方法为传统的,对生态有害的生产途径提供了可行的替代方案.
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