猪成熟卵细胞中母性因素的空间分布
Fuquan Zhu1, Meng Yang1, Dayu Wang1
1College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Animal biotechnology
|August 26, 2024
概括
在蛋白质水平上检测到母体因子在猪卵细胞中的空间不对称性. 研究人员确定了参与细胞循环和翻译的差异表达蛋白质,为早期胚胎发育提供了新的见解.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 不对称的母体转录对于早期胚胎细胞命运至关重要.
- 关于哺乳动物卵细胞,特别是猪卵细胞中的母性因素的研究有限.
研究的目的:
- 为了研究猪卵细胞中的空间因素.
- 在质细胞和细胞质细胞之间识别差异表达蛋白 (DEP).
主要方法:
- 面向切割技术用于有效收集质细胞 (NSO) 和细胞质细胞 (SSO).
- RNA测序 (RNA-Seq) 和液体染色学-质谱/质谱 (LC-MS/MS) 分析.
- 生物信息分析用于识别差异表达基因 (DEGs) 和蛋白质 (DEPs).
主要成果:
- 在NSO和SSO之间没有发现差异表达基因 (DEG).
- 鉴定了89种差异表达蛋白 (DEP);与SSO相比,在NSO中有58种高调和31种下调.
- DEP主要参与细胞循环和核糖体通路. 上调的DEP与螺旋和翻译调节有关,而下调的DEP与细胞质小核糖体子单元和mRNA结合有关.
- 参与基因表达调节和表观遗传学的蛋白质SIRT5在NSO中被上调.
结论:
- 孕产妇因子在蛋白质水平上的空间不对称性首次在成熟的猪卵细胞中被证明.
- 这些发现为蛋白质局部化在早期胚胎发育和猪细胞命运决定中的作用提供了关键的见解.
相关概念视频
02:07Oogenesis
63.6K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
63.6K
01:20Folliculogenesis
670
Folliculogenesis is the development of ovarian follicles, the specialized structures within the ovarian cortex where oogenesis, or egg development, occurs. This process is essential for female reproductive health and begins during fetal development when primordial follicles are formed. Each primordial follicle comprises a primary oocyte in the center, surrounded by a single layer of squamous pre-granulosa cells. These follicles remain dormant in late prophase I of meiosis until triggered by...
670